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Npntem1(flpo)Ngai
Endonuclease-mediated Allele Detail
Summary
Symbol: Npntem1(flpo)Ngai
Name: nephronectin; endonuclease-mediated mutation 1, John Ngai
MGI ID: MGI:6865658
Gene: Npnt  Location: Chr3:132587506-132656052 bp, - strand  Genetic Position: Chr3, 61.52 cM
Alliance: Npntem1(flpo)Ngai page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:325783
Parent Cell Line:  v6.5 (ES Cell)
Strain of Origin:  (C57BL/6 x 129S4/SvJae)F1
Mutation
description
Allele Type:    Endonuclease-mediated (Recombinase)
Mutation:    Insertion
 
Npntem1(flpo)Ngai expression driven by 1 gene
 
Mutation details: Using CRISPR/Cas9 technology, the P2A-FlpO sequence (encoding a viral 2A oligopeptide that mediates ribosomal skipping and a codon-optimized FLPe gene modified for translation in mammalian cells and higher recombination efficiency in cells and in mice) was inserted after the STOP codon of the nephronectin gene (Npnt) on chromosome 3 in v6.5 embryonic stem (ES) cells. Chimeric mice were generated by aggregating ES cells with morula-stage embryos. (J:325783)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Npnt (mouse)
Summary of all recombinase alleles driven by Npnt.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Npnt Mutation:  43 strains or lines available
References
Original:  J:325783 A multimodal cell census and atlas of the mammalian primary motor cortex. Nature. 2021 Oct;598(7879):86-102
All:  1 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory