Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:35169
Chazaud C, et al., Restricted expression of a novel retinoic acid responsive gene during limb bud dorsoventral patterning and endochondral ossification. Dev Genet. 1996;19(1):66-73
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:6867832
|
Gene symbol:
|
Stra6
|
Gene name:
|
stimulated by retinoic acid gene 6
|
Probe:
|
Stra6 cDNA2
|
Probe preparation:
|
Antisense labelled with S35 RNA
|
Visualized with:
|
Autoradiography
|
|
Results
|
Specimen
not shown:
embryonic day 8.5-9.0
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 8.5-9.0
|
Sex:
|
Not Specified
|
Type:
|
Not Specified
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
1A:
embryonic day 9.5
(more )
Specimen
1A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: forelimb bud mesenchyme |
Present |
Regionally restricted |
1A
|
Expression was detected in the proximal and dorsal side of the forelimb bud. Transcript levels were stronger in the proximal regions and gradually decreased toward the distal margin.
|
TS15: trunk mesenchyme |
Present |
Regionally restricted |
1A
|
Expression was detected in the flank mesenchyme prior to the appearance of the hindlimb bud.
|
Specimen
1B:
embryonic day 10.0
(more )
Specimen
1B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: trunk mesenchyme |
Present |
Regionally restricted |
1B
|
Expression was continuous in the flank mesenchyme between the fore- and hindlimb buds.
|
Specimen
1C:
embryonic day 10.0
(more )
Specimen
1C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: hindlimb bud mesenchyme |
Present |
Regionally restricted |
1C Stra6
|
Expression was restricted to the posterior and proximal mesoderm in the hindlimbs.
|
TS16: forelimb bud mesenchyme |
Present |
Regionally restricted |
1C Stra6
|
Expression was detected along the dorsal mesoderm of the forelimb buds except in the most distal region or progress zone.
|
Specimen
1D:
embryonic day 11.5
(more )
Specimen
1D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: forelimb bud mesenchyme |
Present |
Regionally restricted |
1D Stra6
|
Expression was restricted to the superficial limb mesenchyme, both dorsally and ventrally, and excluded from the central chondrogenic (precartilaginous) condensations. Expression was not detected in the distal tip. Expression was outside of the progress zone.
|
Specimen
3A:
embryonic day 13.5
(more )
Specimen
3A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3B:
embryonic day 13.5
(more )
Specimen
3B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3C:
embryonic day 16.5
(more )
Specimen
3C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: perichondrium |
Present |
Regionally restricted |
3C
|
Expression was restricted to very limited concentric zones of the hindlimb perichondrium.
|
TS24: hindlimb muscle |
Weak |
|
3C
|
|
Specimen
3D:
embryonic day 16.5
(more )
Specimen
3D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: perichondrium |
Present |
Regionally restricted |
3D
|
Expression was restricted to very limited concentric zones of the femur perichondrium. The labeled areas were located slightly distally to the front of hypertrophic chondrocytes and overlying the cartilage 'elongation zone'.
|
|