Gene Expression Data
Assay Details
Assay
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Reference:
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J:35169
Chazaud C, et al., Restricted expression of a novel retinoic acid responsive gene during limb bud dorsoventral patterning and endochondral ossification. Dev Genet. 1996;19(1):66-73
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6867833
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Gene symbol:
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Stra6
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Gene name:
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stimulated by retinoic acid gene 6
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Probe:
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Stra6 cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
2A:
embryonic day 10.5
(more )
Specimen
2A:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: trunk mesenchyme |
Present |
Regionally restricted |
2A
|
Expression was detected in the flank mesenchyme. The domain appeared to be continuous with the limb bud dorsal expression domains and was restricted to a relatively narrow stripe.
|
TS17: forelimb bud |
Present |
Regionally restricted |
2A
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Expression was detected along the dorsal and ventral sides of the forelimb buds.
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TS17: hindlimb bud |
Present |
Regionally restricted |
2A
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Expression was detected along the dorsal side of the hindlimb buds.
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TS17: dorsal root ganglion |
Present |
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2A
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Specimen
2B:
embryonic day 10.5
(more )
Specimen
2B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: trunk mesenchyme |
Present |
Regionally restricted |
2B
|
Expression was detected in the flank mesenchyme. The domain appeared to be continuous with the limb bud dorsal expression domains and was restricted to a relatively narrow stripe.
|
TS17: forelimb bud |
Present |
Regionally restricted |
2B
|
Expression was detected along the dorsal and ventral sides of the forelimb buds. Both the dorsal and ventral domains extended toward the proximal aspect of the limbs, but did not reach their distal extremities.
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TS17: hindlimb bud mesenchyme |
Present |
Regionally restricted |
2B
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Expression remained restricted to the posterior and proximal mesenchyme of the hindlimb.
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TS17: dorsal root ganglion |
Present |
|
2B
|
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Specimen
2C:
embryonic day 11.0
(more )
Specimen
2C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.0
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Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: hindlimb bud |
Present |
Regionally restricted |
2C
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A subtle ventral expression domain was detected in the hindlimb bud.
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TS18: forelimb bud |
Present |
Regionally restricted |
2C
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Two proximal expression domains were detected along the dorsal side of the forelimb bud.
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TS18: dorsal root ganglion |
Present |
|
2C
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TS18: face mesenchyme |
Present |
|
2C
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TS18: trunk mesenchyme |
Present |
Regionally restricted |
2C
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Expression was detected in the flank mesenchyme. The domain appeared to be continuous with the limb bud dorsal expression domains and was restricted to a relatively narrow stripe.
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Specimen
2D:
embryonic day 10.5
(more )
Specimen
2D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: trunk mesenchyme |
Present |
Regionally restricted |
2D
|
Expression was detected in the flank mesenchyme.
|
TS17: forelimb bud |
Present |
Regionally restricted |
2D
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An additional domain of expression was detected along the ventral margin of the forelimb.
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Specimen
2E:
embryonic day 10.5
(more )
Specimen
2E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: forelimb bud |
Present |
Regionally restricted |
2E
|
Expression was detected along the dorsal and ventral sides of the forelimb buds. Both the dorsal and ventral domains extended toward the proximal aspect of the limbs, but did not reach their distal extremities.
|
Specimen
2F:
embryonic day 10.5
(more )
Specimen
2F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: trunk mesenchyme |
Present |
Regionally restricted |
2F
|
Expression was detected in the flank. mesenchyme. The domain appeared to be continuous with the limb bud dorsal expression domains and was restricted to a relatively narrow stripe.
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