Col1a2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:308829 Yamaguchi H, et al., The molecular complex of ciliary and golgin protein is crucial for skull development. Development. 2021 Jul 1;148(13):dev199559
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7261150
Gene symbol:	Col1a2
Gene name:	collagen, type I, alpha 2

Antibody:	anti-collagen type I (CL50151AP)
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

1D ©

Specimen 1D: embryonic day 18.5 (more

)

Structure	Level	Pattern	Image	Note
TS26: cranium	Present		1D	Expression in osteoblasts near osteogenic front

1Da ©

1Da inset ©

Specimen 1Da: embryonic day 18.5 (more

)
Note: Triple labeled: green - Col1a1 and Col1a2; magenta - Golga2 (GM130; assay MGI:7260357); blue - DAPI.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: cranium	calvarial osteoblast	Present		1Da inset , 1Da

1Db ©

1Db inset ©

Specimen 1Db: embryonic day 18.5 (more

)
Note: Triple labeled: green - Col1a1 and Col1a2; magenta - Golga2 (GM130; assay MGI:7260357); blue - DAPI.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: cranium	calvarial osteoblast	Present		1Db inset , 1Db

1Dc ©

1Dc inset ©

Specimen 1Dc: embryonic day 18.5 (more

)
Note: Triple labeled: green - Col1a1 and Col1a2; magenta - Golga2 (GM130; assay MGI:7260357); blue - DAPI.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: cranium	calvarial osteoblast	Present		1Dc , 1Dc inset

1Dd ©

1Dd inset ©

Specimen 1Dd: embryonic day 18.5 (more

)
Note: Triple labeled: green - Col1a1 and Col1a2; magenta - Golga2 (GM130; assay MGI:7260357); blue - DAPI.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: cranium	calvarial osteoblast	Present		1Dd , 1Dd inset

2D WT ©

Specimen 2D WT: embryonic day 18.5 (more

)
Note: Super-resolution microscopy.

Structure	Level	Pattern	Image	Note
TS26: cranium	Present	Regionally restricted	2D WT	Expression in fibrils

2D WT inset ©

Specimen 2D WT inset: embryonic day 18.5 (more

)
Note: Super-resolution microscopy.

Structure	Level	Pattern	Image	Note
TS26: cranium	Present	Regionally restricted	2D WT inset	Expression in fibrils

2D Ift20 cKO ©

Specimen 2D Ift20 cKO: embryonic day 18.5; H2az2^{Tg(Wnt1-cre)11Rth}/H2az2⁺, Ift20^tm1.1Gjp/Ift20^tm1.1Gjp (more

)
Note: Conditional mutant. Super-resolution microscopy.

Structure	Level	Pattern	Image	Note
TS26: cranium	Weak	Regionally restricted	2D Ift20 cKO	Number of collagen fibrils reduced compared with controls.

Specimen 2D Ift20 cKO inset: embryonic day 18.5; H2az2^{Tg(Wnt1-cre)11Rth}/H2az2⁺, Ift20^tm1.1Gjp/Ift20^tm1.1Gjp (more

)
Note: Conditional mutant. Super-resolution microscopy.

Structure	Level	Pattern	Image	Note
TS26: cranium	Weak	Regionally restricted	2D Ift20 cKO inset	Number of collagen fibrils reduced compared with controls.

S5A WT ©

Specimen S5A WT: embryonic day 18.5 (more

)

Structure	Level	Pattern	Image	Note
TS26: cranium	Present		S5A WT	Normal expression in both control and cKO skulls.

Specimen S5A Trip11 CKO: embryonic day 18.5; H2az2^{Tg(Wnt1-cre)11Rth}/H2az2⁺, Trip11^tm1Psmi/Trip11^tm1Psmi (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS26: cranium	Present		S5A Trip11 cKO	Normal expression in both control and cKO skulls.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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