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Gene Expression Data
Assay Details
Assay
Reference: J:132315 Lynn FC, et al., MicroRNA expression is required for pancreatic islet cell genesis in the mouse. Diabetes. 2007 Dec;56(12):2938-45
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7266729
Gene symbol: Gcg
Gene name: glucagon
Antibody: anti-glucagon
Detection system: Secondary antibody coupled to Cy3
Assay notes: Two antibodies were described, but the one used in this assay was not detailed. Anti-glucagon MGI:7266699 may have been used in this assay.
Results
Specimen 3C: embryonic day 18.5; Dicer1tm1Bdh/Dicer1+, Tg(Pdx1-cre)89.1Dam/0 (more )
Note: Conditional mutant. Double labeled: red - Gcg; green - Ins1 (assay MGI:7266727) and Ins2 (assay MGI:7266727).
Structure Cell Type Level Pattern Image Note
TS26: endocrine pancreas pancreatic A cell Present 3C
Specimen 3D: embryonic day 18.5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Pdx1-cre)89.1Dam/0 (more )
Note: Conditional mutant. Double labeled: red - Gcg; green - Ins1 (assay MGI:7266727) and Ins2 (assay MGI:7266727).
Structure Cell Type Level Pattern Image Note
TS26: endocrine pancreas pancreatic A cell Weak 3D Decrease (79%) in alpha cells compared with control.
Specimen 4A: postnatal month 8 (more )
Note: Double labeled: red - Gcg; green - Ins1 (assay MGI:7266727) and Ins2 (assay MGI:7266727).
Structure Cell Type Level Pattern Image Note
TS28: endocrine pancreas pancreatic A cell Present 4A
Specimen 4B: postnatal month 8; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Ins2-cre)25Mgn/0 (more )
Note: Conditional mutant. Double labeled: red - Gcg; green - Ins1 (assay MGI:7266727) and Ins2 (assay MGI:7266727).
Structure Cell Type Level Pattern Image Note
TS28: endocrine pancreas pancreatic A cell Present 4B Normal expression compared with control.
Specimen 5A: embryonic day 12.5; Dicer1tm1Bdh/Dicer1+, Tg(Pdx1-cre)89.1Dam/0 (more )
Note: Conditional mutant. Double labeled: red - Gcg; green - Pdx1 (assay MGI:7266723).
Structure Level Pattern Image Note
TS20: pancreas Present 5A Expression in the earliest differentiated cells.
Specimen 5B: embryonic day 12.5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Pdx1-cre)89.1Dam/0 (more )
Note: Conditional mutant. Double labeled: red - Gcg; green - Pdx1 (assay MGI:7266723).
Structure Level Pattern Image Note
TS20: pancreas Present 5B No difference compared with control.
Specimen 5E: embryonic day 15.5; Dicer1tm1Bdh/Dicer1+, Tg(Pdx1-cre)89.1Dam/0 (more )
Note: Conditional mutant. Double labeled: red - Gcg; green - Neurog3 (assay MGI:7266736).
Structure Level Pattern Image Note
TS23: endocrine pancreas Present 5E
Specimen 5F: embryonic day 15.5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Pdx1-cre)89.1Dam/0 (more )
Note: Conditional mutant. Double labeled: red - Gcg; green - Neurog3 (assay MGI:7266736).
Structure Level Pattern Image Note
TS23: endocrine pancreas Present 5F No significant decrease in expression compared with control.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/17/2024
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