Rest Western blot Gene Expression Assay - GXD

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Gene Expression Data
Assay Details

Assay

Reference:	J:322393 Kimura R, et al., Detection of REST expression in the testis using epitope-tag knock-in mice generated by genome editing. Dev Dyn. 2022 Mar;251(3):525-535
Assay type:	Western blot
MGI Accession ID:	MGI:7275279
Gene symbol:	Rest
Gene name:	RE1-silencing transcription factor

Antibody:	anti-REST (07-579)
Detection system:	Secondary antibody coupled to horseradish peroxidase

Results

Image: 3D anti-REST (07-579) ©

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	200 kDa (a)	Genetic Background	Mutant Allele(s)	Sex
Rest+/+ 1	P newborn	TS27: testis	Present	involves: C57BL/6J		Male
Rest+/+ 2	P newborn	TS27: testis	Present	involves: C57BL/6J		Male
Rest+/+ 3	P newborn	TS27: testis	Present	involves: C57BL/6J		Male
RestPA/PA 1	P newborn	TS27: testis	Present	involves: C57BL/6J	Rest^em1Nosu/Rest^em1Nosu	Male
RestPA/PA 2	P newborn	TS27: testis	Present	involves: C57BL/6J	Rest^em1Nosu/Rest^em1Nosu	Male
RestPA/PA 3	P newborn	TS27: testis	Present	involves: C57BL/6J	Rest^em1Nosu/Rest^em1Nosu	Male

Notes:
(a) Multiple bands including the ~200 kDa band corresponding with Rest were detected.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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