Probe:	Neph1 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: branchial arch	Present		1A
TS15: dorsal aorta	Present		1A
TS15: olfactory placode	Present		1A
TS15: sclerotome	Present		1A
TS15: future brain	Present		1A
TS15: otocyst	Present	Regionally restricted	1A	Expressed around the otic vesicle.
TS15: forelimb bud	Present	Uniform	1A
TS15: hindlimb ridge	Present	Uniform	1A
TS15: heart atrium	Weak		1A
TS15: primitive ventricle	Weak		1A

Structure	Level	Pattern	Image	Note
TS17: otocyst	Absent		1B
TS17: telencephalon	Strong	Regionally restricted	1B	Expressed in the telocele.
TS17: branchial arch	Present		1B
TS17: olfactory placode	Present		1B
TS17: sclerotome	Present		1B
TS17: forelimb bud	Present	Uniform	1B
TS17: hindlimb bud	Present	Uniform	1B
TS17: heart atrium	Present		1B	Increased expression.
TS17: primitive ventricle	Present		1B	Increased expression.

Structure	Level	Pattern	Image	Note
TS19: otocyst	Absent		1C
TS19: telencephalon	Strong	Regionally restricted	1C	Expressed in the telocele.
TS19: forelimb bud	Present		1C
TS19: hindlimb bud	Present		1C
TS19: olfactory epithelium	Present		1C
TS19: sclerotome	Present		1C
TS19: footplate apical ectodermal ridge	Present		1C	Increased level.
TS19: handplate apical ectodermal ridge	Present		1C	Increased level.
TS19: heart atrium	Present		1C	Increased expression.
TS19: heart left ventricle	Present		1C	Increased expression.
TS19: hindbrain	Trace		1C	Almost void of expression.
TS19: midbrain	Trace		1C	Almost void of expression.

Structure	Level	Pattern	Image	Note
TS19: branchial arch	Present		2A	Almost ubiquitous expression with locally increased intensity.
TS19: olfactory epithelium	Present		2A	Almost ubiquitous expression with locally increased intensity.

Structure	Level	Pattern	Image	Note
TS22: inferior colliculus	Present		2B
TS22: superior colliculus	Present		2B
TS22: spinal cord	Weak	Regionally restricted	2B	Expressed in two tracts of cells spanned the spinal cord in a cranio-caudal direction.
TS22: midbrain tegmentum	Present	Regionally restricted	2B	Expressed in small domains.
TS22: thalamus	Present	Regionally restricted	2B	Expressed in small domains.

Structure	Level	Pattern	Image	Note
TS20: adenohypophysis	Present		2C	anterior pituitary lobe
TS20: neurohypophysis pars nervosa	Present	Regionally restricted	2C	Expressed in the base of the third ventricle in the neuroepithelium of the developing posterior lobe.

Structure	Level	Pattern	Image	Note
TS20: bronchus epithelium	Present		2D

Structure	Level	Pattern	Image	Note
TS24: neocortex	Present		2E

Structure	Level	Pattern	Image	Note
TS24: hippocampus	Present		2F

Structure	Level	Pattern	Image	Note
TS24: ganglionic eminence	Present		2G