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Chodlem1(cre)Ngai
Endonuclease-mediated Allele Detail
Summary
Symbol: Chodlem1(cre)Ngai
Name: chondrolectin; endonuclease-mediated mutation 1, John Ngai
MGI ID: MGI:7286049
Gene: Chodl  Location: Chr16:78727836-78748621 bp, + strand  Genetic Position: Chr16, 45.39 cM, cytoband C3.2
Alliance: Chodlem1(cre)Ngai page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:101977
Parent Cell Line:  v6.5 (ES Cell)
Strain of Origin:  (C57BL/6 x 129S4/SvJae)F1
Mutation
description
Allele Type:    Endonuclease-mediated (Epitope tag, Recombinase)
Mutation:    Insertion
 
Chodlem1(cre)Ngai expression driven by 1 gene
 
Mutation details: Using CRISPR/Cas9 technology, a P2A-Cre (encoding a viral 2A oligopeptide that mediates ribosomal skipping and a codon-optimized cre gene) and a myc-tag were inserted into the targetted STOP codon of the Chondrolectin gene (Chodl) on chromosome 16 in embryonic stem cells. Chimeric mice were generated by aggregating ES cells with morula-stage embryos and bred for germline transmission.The ampicillin cassette is still present in this allele. (J:101977)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Chodl (mouse)
Summary of all recombinase alleles driven by Chodl.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Chodl Mutation:  25 strains or lines available
References
Original:  J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017;
All:  1 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
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