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Cplx3em1(flpo*)Ngai
Endonuclease-mediated Allele Detail
Summary
Symbol: Cplx3em1(flpo*)Ngai
Name: complexin 3; endonuclease-mediated mutation 1, John Ngai
MGI ID: MGI:7286055
Gene: Cplx3  Location: Chr9:57507275-57513564 bp, - strand  Genetic Position: Chr9, 31.11 cM
Alliance: Cplx3em1(flpo*)Ngai page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:101977
Parent Cell Line:  v6.5 (ES Cell)
Strain of Origin:  (C57BL/6 x 129S4/SvJae)F1
Mutation
description
Allele Type:    Endonuclease-mediated (Recombinase)
Mutation:    Insertion
 
Cplx3em1(flpo*)Ngai expression driven by 1 gene
 
Mutation details: Using CRISPR/Cas9 technology, a P2A-Flpo (encoding a viral 2A oligopeptide that mediates ribosomal skipping and a codon-optimized FLPe gene modified for translation in mammalian cells and higher recombination efficiency in cells and in mice) was inserted into the targetted STOP codon of the Complexin-3 gene (Cplx3) on chromosome 9 in embryonic stem cells. This flpo coding sequence was further codon modified; standard flpo PCR primers may not anneal. Chimeric mice were generated by aggregating ES cells with morula-stage embryos and bred for germline transmission.The ampicillin cassette is still present in this allele. (J:101977)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Cplx3 (mouse)
Summary of all recombinase alleles driven by Cplx3.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Cplx3 Mutation:  9 strains or lines available
References
Original:  J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017;
All:  1 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
11/19/2024
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