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Sp7em1(flpo)Oam
Endonuclease-mediated Allele Detail
Summary
Symbol: Sp7em1(flpo)Oam
Name: Sp7 transcription factor 7; endonuclease-mediated mutation 1, Ormond A MacDougald
MGI ID: MGI:7294193
Synonyms: Osterix-FLPo
Gene: Sp7  Location: Chr15:102265038-102275498 bp, - strand  Genetic Position: Chr15, 57.51 cM
Alliance: Sp7em1(flpo)Oam page
Mutation
origin
Strain of Origin:  C57BL/6 x SJL
Mutation
description
Allele Type:    Endonuclease-mediated (Recombinase)
Mutation:    Insertion
 
Sp7em1(flpo)Oam expression driven by 1 gene
 
Mutation details: CRISPR/cas9 endonuclease-mediated genome editing was used to insert an in frame protein containing Sp7-P2A-Flpo sequence- polyA into the 3' UTR of the gene The self-cleaving P2A sequence allows for expression of Sp7 and flpo recombinase. Cas9 protein, sgRNA (gatctgagctgggtagaggaagg), and ssDNA donor were mixed and injected into the pronucleus of C57BL/6J x SJL fertilized zygotes. (J:325967)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Sp7 (mouse)
Summary of all recombinase alleles driven by Sp7.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Sp7 Mutation:  21 strains or lines available
References
Original:  J:325967 Li Z, et al., Lipolysis of bone marrow adipocytes is required to fuel bone and the marrow niche during energy deficits. Elife. 2022 Jun 22;11:e78496
All:  3 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
12/10/2024
MGI 6.24 		The Jackson Laboratory