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Gt(ROSA)26Sortm11(flpo)Amc
Targeted Allele Detail
Summary
Symbol: Gt(ROSA)26Sortm11(flpo)Amc
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 11, Andrew P McMahon
MGI ID: MGI:7327519
Synonyms: Rosa26 (FLPo)
Gene: Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance: Gt(ROSA)26Sortm11(flpo)Amc page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:327127
Parent Cell Line:  v6.5 (ES Cell)
Strain of Origin:  (C57BL/6 x 129S4/SvJae)F1
Mutation
description
Allele Type:    Targeted (Conditional ready, RMCE-ready, Recombinase)
Mutation:    Insertion
 
Gt(ROSA)26Sortm11(flpo)Amc expression driven by 1 gene
 
Mutation detailsThe following elements were inserted into the locus to create an RMCE (recombinase-mediated cassette exchange)-ready allele: a splice acceptor site, an ATG translation start site, an F3 site, a puromycin resistance gene cassette, a flpo recombinase gene cassette (with PGK promoter and poly(A) signal sequence), an FRT site and chicken beta-globin locus insulator sequence. When cells containing this allele are presented with a compatible RMCE vector, the sequence between the F3-FRT sites will be replaced with the sequence between the F3-FRT sites in the vector. (J:327127)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: PGK1 (species not specified)
Summary of all recombinase alleles driven by PGK1.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  994 strains or lines available
References
Original:  J:327127 Tsanov KM, et al., An embryonic stem cell-based system for rapid analysis of transcriptional enhancers. Genesis. 2012 May;50(5):443-50
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
12/17/2024
MGI 6.24
The Jackson Laboratory