Gene Expression Data
Assay Details
Assay
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Reference:
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J:185804
Larkins CE, et al., Defective Nodal and Cerl2 expression in the Arl13b(hnn) mutant node underlie its heterotaxia. Dev Biol. 2012 Jul 1;367(1):15-24
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7331400
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Gene symbol:
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Dand5
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Gene name:
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DAN domain family member 5, BMP antagonist
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Results
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Specimen
5D:
embryonic day 7.5
(more )
Note: presomite stage
Specimen
5D:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
|
Note:
|
presomite stage
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: node |
Present |
|
5D
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Right-sided asymmetry was seen (10 out of 19 embryos).
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Specimen
5E:
(close )
Genetic Background:
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C3.B6-Arl13bhnn
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Mutant Allele(s):
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Arl13bhnn/Arl13bhnn
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Age:
|
embryonic day 7.5
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
presomite stage
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: node |
Present |
|
5E
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Slightly higher levels of expression in the left side (4 out 8 embryos).
|
Specimen
not shown:
embryonic day 7.5
(more )
Note: presomite stage
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
presomite stage
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: node |
Present |
|
|
No asymmetry was seen in 5 out of 19 embryos; left-sided in 4 (summarized in 5D).
|
Specimen
not shown:
(close )
Genetic Background:
|
C3.B6-Arl13bhnn
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Mutant Allele(s):
|
Arl13bhnn/Arl13bhnn
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
presomite stage
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: node |
Present |
|
|
Right-sided asymmetry was seen in 2 out of 8 embryos; no asymmetry in 2 (summarized in 5E).
|
Specimen
not shown:
embryonic day 8.0
(more )
Note: 2-3 somites stage
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
2-3 somites stage
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: node |
Present |
|
|
Right-sided asymmetry was seen in 25 out of 26 embryos; left-sided in 1 (summarized in 5D).
|
Specimen
not shown:
embryonic day 8.0; Arl13bhnn/ Arl13bhnn
(more )
Note: 2-3 somites stage
Specimen
not shown:
(close )
Genetic Background:
|
C3.B6-Arl13bhnn
|
Mutant Allele(s):
|
Arl13bhnn/Arl13bhnn
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Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
2-3 somites stage
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: node |
Present |
|
|
Symmetric expression was seen in 2 out of 4 embryos, left-sided in 1, and right-sided in 1 (summarized in 5E).
|
Specimen
not shown:
embryonic day 8.0
(more )
Note: 4-5 somites stage
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
4-5 somites stage
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: node |
Present |
|
|
Right-sided asymmetry was seen in 15 out of 16 embryos, symmetric expression in 1 (summarized in 5D).
|
Specimen
not shown:
embryonic day 8.0; Arl13bhnn/ Arl13bhnn
(more )
Note: 4-5 somites stage
Specimen
not shown:
(close )
Genetic Background:
|
C3.B6-Arl13bhnn
|
Mutant Allele(s):
|
Arl13bhnn/Arl13bhnn
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
4-5 somites stage
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: node |
Present |
|
|
Symmetric expression was seen in 5 out of 6 embryos, right-sided in 1 (summarized in 5E).
|
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