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Gene Expression Data
Assay Details
Assay
Reference: J:241343 Furuya S, et al., Inactivation of the 3-phosphoglycerate dehydrogenase gene in mice: changes in gene expression and associated regulatory networks resulting from serine deficiency. Funct Integr Genomics. 2008 Aug;8(3):235-49
Assay type: RT-PCR
MGI Accession ID: MGI:7335986
Gene symbol: Cars1
Gene name: cysteinyl-tRNA synthetase 1
Probe: Cars-pC, Cars-pD
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR. Relative expression was normalized to Gapdh. Data was used for cross validation of microarray results.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
WT E13.5 TS21: head Present 1 µg; total RNA Not Specified Not Specified
KO E13.5 TS21: head Present (a) 1 µg; total RNA B6.129P2(FVB)-Phgdhtm1.1Shfu Phgdhtm1.1Shfu/Phgdhtm1.1ShfuNot Specified
Notes:
(a) Expression level was similar to that measured with the microarray (upregulated in the mutant).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/10/2024
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