Gene Expression Data
Assay Details
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Assay
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Reference:
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J:39131
Fujii S, et al., Immunohistochemical evaluation of glucocorticoid receptors in developing eyelids of Nc-eob fetuses with genetically determined open-eyelid malformation. Congenit Anom (Kyoto). 1996;36:227-34
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7336144
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Gene symbol:
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Nr3c1
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Gene name:
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nuclear receptor subfamily 3, group C, member 1
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Antibody:
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Anti-GR (PA1-511)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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Fix: 3.5% paraformaldehyde.
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Results
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Specimen
1A:
embryonic day 14.5
(more  )
Specimen
1A:
(close  )
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Genetic Background:
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NC
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
1B:
embryonic day 14.5; eob/ eob
(more )
Specimen
1B:
(close )
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Genetic Background:
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involves: NC
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Mutant Allele(s):
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eob/eob
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: eyelid epithelium |
epithelial cell |
Strong |
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1B
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Strong nuclear localization. No significant difference in staining was noted in the mutant compared with wild type.
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| TS22: eyelid mesenchyme |
mesenchymal cell |
Strong |
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1B
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Strong nuclear localization. No significant difference in staining was noted in the mutant compared with wild type.
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Specimen
1C:
embryonic day 14.5
(more )
Specimen
1C:
(close )
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Genetic Background:
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NC
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
1D:
embryonic day 14.5; eob/ eob
(more )
Specimen
1D:
(close )
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Genetic Background:
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involves: NC
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Mutant Allele(s):
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eob/eob
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: palatal shelf epithelium |
epithelial cell |
Present |
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1D
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No significant difference in staining was noted in the mutant compared with wild type.
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| TS22: palatal shelf mesenchyme |
mesenchymal cell |
Present |
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1D
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No significant difference in staining was noted in the mutant compared with wild type.
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Specimen
2A:
embryonic day 15.5
(more )
Specimen
2A:
(close )
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Genetic Background:
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NC
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2B:
embryonic day 15.5; eob/ eob
(more )
Specimen
2B:
(close )
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Genetic Background:
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involves: NC
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Mutant Allele(s):
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eob/eob
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: eyelid epithelium |
epithelial cell |
Strong |
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2B
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Strong nuclear localization. No significant difference in staining was noted in the mutant compared with wild type.
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| TS23: eyelid mesenchyme |
mesenchymal cell |
Present |
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2B
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Expression level was lower in the mutant compared with wild type.
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Specimen
2C:
embryonic day 15.5
(more )
Specimen
2C:
(close )
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Genetic Background:
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NC
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: secondary palate |
Present |
Regionally restricted |
2C
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Expression was detected in both epithelium and mesenchyme.
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Specimen
2D:
embryonic day 15.5; eob/ eob
(more )
Specimen
2D:
(close )
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Genetic Background:
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involves: NC
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Mutant Allele(s):
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eob/eob
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: secondary palate |
Present |
Regionally restricted |
2D
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Expression was detected in both epithelium and mesenchyme. Expression level was comparable between mutant and wild type.
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Specimen
Table 1 NC:
embryonic day 14.5
(more )
Specimen
Table 1 NC:
(close )
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Genetic Background:
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NC
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
Table 1 NC-eob:
embryonic day 14.5; eob/ eob
(more )
Specimen
Table 1 NC-eob:
(close )
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Genetic Background:
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involves: NC
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Mutant Allele(s):
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eob/eob
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lower eyelid epithelium |
Present |
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No significant differences were observed in the immunostaining density of the mutant compared with wild type.
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| TS22: lower eyelid mesenchyme |
Present |
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No significant differences were observed in the immunostaining density of the mutant compared with wild type.
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| TS22: upper eyelid epithelium |
Present |
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No significant differences were observed in the immunostaining density of the mutant compared with wild type.
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| TS22: upper eyelid mesenchyme |
Present |
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No significant differences were observed in the immunostaining density of the mutant compared with wild type.
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| TS22: palatal shelf epithelium |
Present |
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No significant differences were observed in the immunostaining density of the mutant compared with wild type.
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| TS22: palatal shelf mesenchyme |
Present |
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No significant differences were observed in the immunostaining density of the mutant compared with wild type.
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Specimen
Table 2 NC:
embryonic day 15.5
(more )
Specimen
Table 2 NC:
(close )
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Genetic Background:
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NC
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
Table 2 NC-eob:
embryonic day 15.5; eob/ eob
(more )
Specimen
Table 2 NC-eob:
(close )
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Genetic Background:
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involves: NC
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Mutant Allele(s):
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eob/eob
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
|
Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: lower eyelid epithelium |
Present |
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No significant differences were observed in the epithelium immunostaining density of the mutant compared with wild type.
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| TS23: upper eyelid epithelium |
Present |
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No significant differences were observed in the epithelium immunostaining density of the mutant compared with wild type.
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| TS23: lower eyelid mesenchyme |
Present |
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On E15.5, the immunostaining density in the mesenchyme of the upper and lower eyelids was significantly lower than in the wild type.
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| TS23: upper eyelid mesenchyme |
Present |
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On E15.5, the immunostaining density in the mesenchyme of the upper and lower eyelids was significantly lower than in the wild type.
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| TS23: secondary palate |
Present |
Regionally restricted |
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Expression was detected in both epithelium and mesenchyme of secondary palate. No significant differences were observed in the immunostaining density of the mutant compared with wild type.
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