Gene Expression Data
Assay Details
Assay
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Reference:
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J:332131
Perera SN, et al., Insights into olfactory ensheathing cell development from a laser-microdissection and transcriptome-profiling approach. Glia. 2020 Dec;68(12):2550-2584
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7424937
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Gene symbol:
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Nkd2
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Gene name:
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naked cuticle 2
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Probe:
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Nkd2 cDNA4
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
3 - Nkd2:
embryonic day 16.5
(more )
Note: Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Schwann cells and Tubb3 (magenta) to identify axons.
Specimen
3 - Nkd2:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Schwann cells and Tubb3 (magenta) to identify axons.
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Specimen
S11 - E11.5:
embryonic day 11.5
(more )
Note: Parasagittal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Schwann cells and Tubb3 (magenta) to identify axons.
Specimen
S11 - E11.5:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Parasagittal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Schwann cells and Tubb3 (magenta) to identify axons.
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS20: olfactory nerve |
olfactory ensheathing cell |
Absent |
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S11B
,
S11C
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The mucosal OECs lack expression.
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Specimen
S11 - E13 - D1:
embryonic day 13.0
(more )
Note: Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Schwann cells and Tubb3 (magenta) to identify axons.
Specimen
S11 - E13 - D1:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 13.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Schwann cells and Tubb3 (magenta) to identify axons.
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Specimen
S11 - E13 - D2:
embryonic day 13.0
(more )
Note: Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Schwann cells and Tubb3 (magenta) to identify axons.
Specimen
S11 - E13 - D2:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 13.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Schwann cells and Tubb3 (magenta) to identify axons.
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Specimen
S13E:
embryonic day 16.5
(more )
Note: Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
Specimen
S13E:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
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Specimen
S13F:
embryonic day 16.5; Ptprz1tm1Mno/ Ptprz1tm1Mno
(more )
Note: Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
Specimen
S13F:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ptprz1tm1Mno/Ptprz1tm1Mno
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
Note:
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Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS24: main olfactory bulb olfactory nerve layer |
olfactory ensheathing cell |
Strong |
Non-Uniform |
S13F1
,
S13F
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The inner ONL-OECs show strong expression, the outer ONL-OECs weaker expression. Expression levels in the mutant are comparable to those seen in wild type.
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Specimen
S14M:
embryonic day 16.5
(more )
Note: Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
Specimen
S14M:
(close )
Genetic Background:
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involves: 129S2/SvPas
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
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Specimen
S14N:
embryonic day 16.5; Ptntm1Tmu/ Ptntm1Tmu
(more )
Note: Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
Specimen
S14N:
(close )
Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Ptntm1Tmu/Ptntm1Tmu
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
Note:
|
Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS24: main olfactory bulb olfactory nerve layer |
olfactory ensheathing cell |
Strong |
Non-Uniform |
S14N1
,
S14N
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The inner ONL-OECs show strong expression, the outer ONL-OECs weaker expression. Expression levels in the mutant are comparable to those seen in wild type.
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