Gene Expression Data
Assay Details
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Assay
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Reference:
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J:332131
Perera SN, et al., Insights into olfactory ensheathing cell development from a laser-microdissection and transcriptome-profiling approach. Glia. 2020 Dec;68(12):2550-2584
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7425191
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Gene symbol:
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Gnrh1
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Gene name:
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gonadotropin releasing hormone 1
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Probe:
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Gnrh1 probe4
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
13f:
embryonic day 16.5
(more  )
Note: Double labeled: magenta - Gnrh1; green - Tubb3 (assay MGI:7425016).
Specimen
13f:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: magenta - Gnrh1; green - Tubb3 (assay MGI:7425016).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: olfactory system |
gonadotropin-releasing hormone neuron |
Present |
Single cells |
13f
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Specimen
13g:
embryonic day 16.5
(more )
Note: Double labeled: magenta - Gnrh1; green - Tubb3 (assay MGI:7425016).
Specimen
13g:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: magenta - Gnrh1; green - Tubb3 (assay MGI:7425016).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: forebrain |
gonadotropin-releasing hormone neuron |
Present |
Single cells |
13g
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Specimen
Ptprz1 -/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ptprz1tm1Mno/Ptprz1tm1Mno
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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These data are quantified in Fig. 13h.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: forebrain |
gonadotropin-releasing hormone neuron |
Present |
Single cells |
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There is no significant difference in the number of GnRH neurons inside the forebrain of the mutant compared to wild type.
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Specimen
Ptn +/+:
embryonic day 16.5
(more )
Note: These data are quantified in Fig. S15D.
Specimen
Ptn +/+:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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These data are quantified in Fig. S15D.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: forebrain |
gonadotropin-releasing hormone neuron |
Present |
Single cells |
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Specimen
Ptn +/-:
embryonic day 16.5; Ptntm1Tmu/Ptn +
(more )
Note: These data are quantified in Fig. S15D.
Specimen
Ptn +/-:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Ptntm1Tmu/Ptn+
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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These data are quantified in Fig. S15D.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: forebrain |
gonadotropin-releasing hormone neuron |
Present |
Single cells |
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There is no significant difference in the number of GnRH neurons inside the forebrain of the heterozygote compared to wild type.
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Specimen
Ptn -/-:
embryonic day 16.5; Ptntm1Tmu/ Ptntm1Tmu
(more )
Note: These data are quantified in Fig. S15D.
Specimen
Ptn -/-:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Ptntm1Tmu/Ptntm1Tmu
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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These data are quantified in Fig. S15D.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: forebrain |
gonadotropin-releasing hormone neuron |
Present |
Single cells |
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There is no significant difference in the number of GnRH neurons inside the forebrain of the null compared to wild type.
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Analysis Tools