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Ddx6em1.2Ysa
Endonuclease-mediated Allele Detail
Summary
Symbol: Ddx6em1.2Ysa
Name: DEAD-box helicase 6; endonuclease-mediated mutation 1.2, Yumiko Saga
MGI ID: MGI:7425331
Synonyms: Ddx6delta
Gene: Ddx6  Location: Chr9:44516189-44552028 bp, + strand  Genetic Position: Chr9, 24.84 cM, cytoband B
Alliance: Ddx6em1.2Ysa page
Mutation
origin
Strain of Origin:  (C57BL/6NCrlj x CBA/JNCrlj)F1
Mutation
description
Allele Type:    Endonuclease-mediated (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation details: By using CRISPR/Cas9 technology in ES cells, the exon 5 (ENSMUSE00000216525) was flanked by loxP sequences and was followed by the Pgk-neo cassette flanked by Frt sequences. The Pgk-neo cassette was removed by crossing with Rosa-Flp mice. Cre-mediated recombination removed exon 5. (J:329565)
Phenotypes
Key:
hm homozygous ht heterozygous tg involves transgenes √ phenotype observed
cn conditional genotype  cx complex: > 1 genome feature ot other: hemizygous, indeterminate,... N normal phenotype
Genotype/
Background:
Allelic Composition
Genetic Background
Cell Line(s)
hm1
Ddx6em1.2Ysa/Ddx6em1.2Ysa
involves: C57BL/6NCrlj * CBA/JNCrlj
 
Phenotypes:
Affected Systems
hm1
show or hide all annotated terms
 
embryo
√
abnormal embryo development
√
abnormal rostral-caudal axis patterning
√
caudal body truncation
√
embryonic growth retardation
√
decreased embryo size
√
abnormal primitive streak formation
√
growth/size/body
√
embryonic growth retardation
√
decreased embryo size
√
mortality/aging
√
embryonic lethality during organogenesis, complete penetrance
√
embryonic lethality between implantation and placentation
√
View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation: 2 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Ddx6 Mutation:  61 strains or lines available
References
Original:  J:329565 Kim J, et al., The RNA helicase DDX6 controls early mouse embryogenesis by repressing aberrant inhibition of BMP signaling through miRNA-mediated gene silencing. PLoS Genet. 2022 Oct;18(10):e1009967
All:  1 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
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Send questions and comments to User Support. 		last database update
12/17/2024
MGI 6.24 		The Jackson Laboratory