Tacr1tm1.1(cre)Hze
Targeted Allele Detail
|
|
| Symbol: |
Tacr1tm1.1(cre)Hze |
| Name: |
tachykinin receptor 1; targeted mutation 1.1, Hongkui Zeng |
| MGI ID: |
MGI:7439128 |
| Synonyms: |
Tacr1-T2A-Cre |
| Gene: |
Tacr1 Location: Chr6:82379315-82537085 bp, + strand Genetic Position: Chr6, 35.94 cM, cytoband D1
|
| Alliance: |
Tacr1tm1.1(cre)Hze page
|
|
|
|
| Allele Type: |
|
Targeted (Recombinase) |
| Mutation: |
|
Insertion
|
| |
|
Tacr1tm1.1(cre)Hze expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Tacr1 (MGI:98475) |
|
|
| |
|
Mutation details: A T2A-Cre-Neo sequence was inserted into the 3' of the Tacr1 gene. The targeting vector contained, from 5' to 3', a partial Tacr1 genomic sequence within exon 4, exon 5, Frt3 site, a modified exon 6 with T2A self-cleaving peptide, Cre recombinase, bovine growth hormone polyA sequence, an AttB sequence, a PGK promoter, a prokaryotic gb2 promoter, a neomycin resistance gene, a PGK polyA sequence, a Frt5 site, a mRNA splice acceptor sequence, one domain of the hygromycin resistance gene, a SV40 polyA sequence, and an AttP site, followed by a 3' repetitive Tacr1 arm, lac operon promoter, origin of replication and ampicillin resistance gene. This construct was electroporated into (129S6/SvEvTac x C57BL/6)F1-derived G4 ES cells. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric mice were bred to PhiC31-expressing mice (JAX Stock No. 007743) to remove the AttB/AttP-flanked sequences including the neo.
(J:260362)
|
|
|
|
Activity: |
 Tissue activity of this recombinase allele
|
|
Driver:
|
Tacr1
(mouse)
|
| |
|
|
| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
| Carrying any Tacr1 Mutation: |
41 strains or lines available
|
|
| Original: |
J:260362 Daigle TL, et al., A suite of transgenic driver and reporter mouse lines with enhanced brain cell type targeting and functionality. Cell. 2018 Jul 12;174(2):465-480.e22 |
| All: |
4 reference(s) |
|
Analysis Tools
