Hey2em1(cre/ERT2)Bzsh
Endonuclease-mediated Allele Detail
|
|
| Symbol: |
Hey2em1(cre/ERT2)Bzsh |
| Name: |
hairy/enhancer-of-split related with YRPW motif 2; endonuclease-mediated mutation 1, Bin Zhou |
| MGI ID: |
MGI:7439153 |
| Synonyms: |
Hey2-2A-CreER |
| Gene: |
Hey2 Location: Chr10:30708355-30718779 bp, - strand Genetic Position: Chr10, 17.19 cM, cytoband A4
|
| Alliance: |
Hey2em1(cre/ERT2)Bzsh page
|
|
| Strain of Origin: |
Not Specified
|
|
| Allele Type: |
|
Endonuclease-mediated (Inducible, Recombinase) |
| Inducer: |
|
tamoxifen |
| Mutation: |
|
Insertion
|
| |
|
Hey2em1(cre/ERT2)Bzsh expression driven by
1 gene
Knock-in expression driven by:
|
| |
|
Mutation details: Using CRISPR/Cas9 technology, a cDNA encoding CreERT2 followed by a polyadenylation sequence was inserted into the last coding exon of Hey2 gene. A P2A peptide sequence was included in the targeting vector to link the Hey2 coding region (before translational stop codon) and CreERT2 cDNA, thus allowing expression of both Hey2 and CreERT2 proteins.
(J:263128)
|
|
|
|
Activity: |
 Tissue activity of this recombinase allele
|
|
Driver:
|
Hey2
(mouse)
|
| |
|
|
| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
| Carrying any Hey2 Mutation: |
31 strains or lines available
|
|
| Original: |
J:263128 Tian X, et al., Identification of a hybrid myocardial zone in the mammalian heart after birth. Nat Commun. 2017 Jul 20;8(1):87 |
| All: |
1 reference(s) |
|
Analysis Tools
