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Gene Expression Data
Assay Details
Assay
Reference: J:245698 Lu S, et al., Developmental vascular remodeling defects and postnatal kidney failure in mice lacking Gpr116 (Adgrf5) and Eltd1 (Adgrl4). PLoS One. 2017;12(8):e0183166
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7439177
Gene symbol: Adgrl4
Gene name: adhesion G protein-coupled receptor L4
Reporter: lacZ
Direct Detection
Results
Specimen 2A Brain: postnatal week 8; Adgrl4tm1(KOMP)Vlcg/Adgrl4+ (more )
Note: 0.2%glutaraldehyde.
Structure Level Pattern Image Note
TS28: brain vascular element Present Regionally restricted 2A Brain Expression was in microvessels.
Specimen 2A Kidney: postnatal week 8; Adgrl4tm1(KOMP)Vlcg/Adgrl4+ (more )
Note: 0.2%glutaraldehyde.
Structure Cell Type Level Pattern Image Note
TS28: renal small blood vessel endothelial cell Present Regionally restricted 2A Kidney Expression was in microvessels.
Specimen 2A Heart: postnatal week 8; Adgrl4tm1(KOMP)Vlcg/Adgrl4+ (more )
Note: 0.2%glutaraldehyde.
Structure Level Pattern Image Note
TS28: heart blood vessel Present Regionally restricted 2A Heart Expression was in microvessels.
Specimen 2A Liver: postnatal week 8; Adgrl4tm1(KOMP)Vlcg/Adgrl4+ (more )
Note: 0.2%glutaraldehyde.
Structure Level Pattern Image Note
TS28: liver vascular element Present Regionally restricted 2A Liver Expression was in microvessels.
Specimen 2B Eltd1lacZ/+ top: postnatal adult; Adgrl4tm1(KOMP)Vlcg/Adgrl4+ (more )
Note: 0.2% paraformaldehyde. Double labeled: green - Adgrl4; red - Pecam1 (assay MGI:7439386).
Structure Cell Type Level Pattern Image Note
TS28: glomerular tuft endothelial cell Present 2B Eltd1lacZ/+ top , 2B Merge top , 2B Merge box top Expression co-localized with Pecam1 in the endothelial cells.
Specimen 2B Eltd1lacZ/+ middle: postnatal adult; Adgrl4tm1(KOMP)Vlcg/Adgrl4+ (more )
Note: 0.2% paraformaldehyde. Double labeled: green - Adgrl4; red - Des (assay MGI:7439387).
Structure Cell Type Level Pattern Image Note
TS28: glomerular tuft mesangial cell Absent 2B Eltd1lacZ/+ middle , 2B Merge middle , 2B Merge box middle Expression did not colocalize with Des in the mesangial cells.
Specimen 2B Eltd1lacZ/+ bottom: postnatal adult; Adgrl4tm1(KOMP)Vlcg/Adgrl4+ (more )
Note: 0.2% paraformaldehyde. Double labeled: green - Adgrl4; red - Nphs2 (assay MGI:7439388).
Structure Cell Type Level Pattern Image Note
TS28: glomerular tuft metanephric podocyte Absent 2B Eltd1lacZ/+ bottom , 2B Merge bottom , 2B Merge box bottom Expression did not colocalize with Nphs2 in the podocytes.
Specimen 3B top: embryonic day 10.0-10.5; Adgrl4tm1(KOMP)Vlcg/Adgrl4+ (more )
Note: 0.2% paraformaldehyde. Double labeled: green - Adgrl4; red - Pecam1 (assay MGI:7439386).
Structure Cell Type Level Pattern Image Note
TS16: heart blood vessel cardiac blood vessel endothelial cell Present 3B merge top , 3B Eltd1-lacZ top
Specimen 3B bottom: embryonic day 10.0-10.5; Adgrl4tm1(KOMP)Vlcg/Adgrl4+ (more )
Note: 0.2% paraformaldehyde. Double labeled: green - Adgrl4; red - Pecam1 (assay MGI:7439386).
Structure Cell Type Level Pattern Image Note
TS16: heart blood vessel cardiac blood vessel endothelial cell Present 3B Eltd1-lacZ bottom , 3B merge bottom
Specimen S2B Heart: embryonic day 18.5; Adgrl4tm1(KOMP)Vlcg/Adgrl4+ (more )
Note: 0.2% paraformaldehyde. Double labeled: green - Adgrl4; red - Pecam1 (assay MGI:7439386).
Structure Cell Type Level Pattern Image Note
TS26: heart blood vessel cardiac blood vessel endothelial cell Present S2B Heart higher mag , S2B Heart Eltd1lacZ/+ , S2B Heart Merge
Specimen S2B Lung: embryonic day 18.5; Adgrl4tm1(KOMP)Vlcg/Adgrl4+ (more )
Note: 0.2% paraformaldehyde. Double labeled: green - Adgrl4; red - Pecam1 (assay MGI:7439386).
Structure Cell Type Level Pattern Image Note
TS26: lung vascular element blood vessel endothelial cell Present S2B Lung Eltd1lacZ/+ , S2B Lung higher mag , S2B Lung Merge
Specimen S2B Sk. muscle: embryonic day 18.5; Adgrl4tm1(KOMP)Vlcg/Adgrl4+ (more )
Note: 0.2% paraformaldehyde. Double labeled: green - Adgrl4; red - Pecam1 (assay MGI:7439386).
Structure Cell Type Level Pattern Image Note
TS26: skeletal muscle blood vessel endothelial cell Present Regionally restricted S2B Sk. muscle Eltd1lacZ/+ , S2B Sk. muscle higher mag , S2B Sk. muscle Merge
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/10/2024
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