Nfatc1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:325730 Ahuja N, et al., Endothelial Cyp26b1 restrains murine heart valve growth during development. Dev Biol. 2022 Jun;486:81-95
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7444038
Gene symbol:	Nfatc1
Gene name:	nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 1

Antibody:	anti-Nfatc1 (sc-7294)
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

6A ©

Specimen 6A: embryonic day 15.5 (more

)
Note: Triple labeled: red - Nfatc1; green - Pbx1 (assay MGI:7444030); white - Sox9 (assay MGI:7444046).

Structure	Level	Pattern	Image	Note
TS23: semilunar valve	Present	Regionally restricted	6A	Expression was detected in the valve endothelium.

6B ©

Specimen 6B: embryonic day 15.5; Cyp26b1^em1Oncl/Cyp26b1^em1Oncl (more

)
Note: Triple labeled: red - Nfatc1; green - Pbx1 (assay MGI:7444030); white - Sox9 (assay MGI:7444046).

Structure	Level	Pattern	Image	Note
TS23: semilunar valve	Present	Regionally restricted	6B	Expression was detected in the valve endothelium.

6D ©

Specimen 6D: embryonic day 15.5 (more

)
Note: Double labeled: red - Nfatc1; green - Ret (assay MGI:7444033).

Structure	Level	Pattern	Image	Note
TS23: semilunar valve	Present	Regionally restricted	6D	Expression was detected in the valve endothelium.

6E ©

Specimen 6E: embryonic day 15.5; Cyp26b1^em1Oncl/Cyp26b1^em1Oncl (more

)
Note: Double labeled: red - Nfatc1; green - Ret (assay MGI:7444033).

Structure	Level	Pattern	Image	Note
TS23: semilunar valve	Present	Regionally restricted	6E	Expression was detected in the valve endothelium.

6G ©

Specimen 6G: embryonic day 15.5 (more

)
Note: Double labeled: green - Nfatc1; red - Foxa1 (assay MGI:7444035).

Structure	Level	Pattern	Image	Note
TS23: semilunar valve	Present	Regionally restricted	6G	Expression was detected in the valve endothelium.

6H ©

Specimen 6H: embryonic day 15.5; Cyp26b1^em1Oncl/Cyp26b1^em1Oncl (more

)
Note: Double labeled: green - Nfatc1; red - Foxa1 (assay MGI:7444035).

Structure	Level	Pattern	Image	Note
TS23: semilunar valve	Present	Regionally restricted	6H	Expression was detected in the valve endothelium.

S7A ©

S7A'' ©

Specimen S7A: embryonic day 15.5 (more

)
Note: Triple labeled: red - Nfatc1; green - Pbx1 (assay MGI:7444030); white - Sox9 (assay MGI:7444046).

Structure	Level	Pattern	Image	Note
TS23: aortic valve	Present	Regionally restricted	S7A'' , S7A	Expression was detected in the valve endothelium.

S7B ©

S7B'' ©

Specimen S7B: embryonic day 15.5; Cyp26b1^em1Oncl/Cyp26b1^em1Oncl (more

)
Note: Triple labeled: red - Nfatc1; green - Pbx1 (assay MGI:7444030); white - Sox9 (assay MGI:7444046).

Structure	Level	Pattern	Image	Note
TS23: aortic valve	Present	Regionally restricted	S7B , S7B''	Expression was detected in the valve endothelium. Expression was unaffected in the mutant compared with wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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