Antibody:	Anti-Pln
Detection system:	Secondary antibody coupled to fluorescein
Assay notes:	Sections were fixed in 100% methanol.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: myometrium		Present		1A
TS28: vasculature of uterus		Present	Regionally restricted	1A	Expression was detected in basal lamina of vascular elements.
TS28: uterus lumen	epithelial cell of uterus	Present	Regionally restricted	1A	Expression was detected in basement membrane of the epithelial cells of the residual lumen.

Structure	Level	Pattern	Image	Note
TS11: amnion	Present		1A
TS11: embryo	Present		1A

Structure	Level	Pattern	Image	Note
TS28: decidua	Strong	Regionally restricted	1B	Expression was high in the developing lacunae.
TS28: extracellular matrix	Present	Regionally restricted	1B	Expression was in the extracellular matrix of the decidua.

Structure	Level	Pattern	Image	Note
TS13: embryo	Present		1B
TS13: extraembryonic component	Absent		1B

Structure	Level	Pattern	Image	Note
TS19: embryo	Present	Regionally restricted	1C	Expression was detected in basal lamina throughout the embryo.
TS19: liver	Present	Regionally restricted	1C	Expression was seen in the liver defining vascular elements.
TS19: amnion	Strong		1C
TS19: heart	Present		1C
TS19: tongue	Present		1C
TS19: brain	Present	Regionally restricted	1C	Expression was detected in regions of the brain, such as the choroid plexus.

Structure	Level	Pattern	Image	Note
TS23: rib cartilage	Present		1D
TS23: cartilage	Present		1D

Structure	Level	Pattern	Image	Note
TS21: intervertebral disc	Absent		2A

Structure	Level	Pattern	Image	Note
TS21: intervertebral disc	Present		2C
TS21: tissue	Present	Regionally restricted	2C	Expression was detected in basal lamina of surrounding tissues.

Structure	Level	Pattern	Image	Note
TS22: intervertebral disc	Present		2E
TS22: tissue	Present	Regionally restricted	2E	Expression was detected in basal lamina of the surrounding tissues.

Structure	Level	Pattern	Image	Note
TS23: cartilage condensation	Present		4A
TS23: tissue	Present	Regionally restricted	4A	Expression was detected in basal lamina of surrounding tissues.

Structure	Level	Pattern	Image	Note
TS19: cartilage condensation	Absent		5B

Structure	Level	Pattern	Image	Note
TS20: cartilage condensation	Weak		5D

Structure	Level	Pattern	Image	Note
TS23: cartilage condensation	Present		6A