Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:309436
Parish EV, et al., Expression of Barhl2 and its relationship with Pax6 expression in the forebrain of the mouse embryo. BMC Neurosci. 2016 Nov 25;17(1):76
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:7461434
|
Gene symbol:
|
Pax6
|
Gene name:
|
paired box 6
|
|
Results
|
Specimen
1A:
embryonic day 8.5
(more )
Note: caudal
Specimen
1A:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
caudal
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: future prosencephalon |
Present |
Regionally restricted |
1A
|
Expressed throughout most of the alar diencephalon, but was absent from the basal plate (summarized in Fig 1M).
|
Specimen
1B:
embryonic day 8.5
(more )
Note: central
Specimen
1B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
central
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: future prosencephalon |
Present |
Regionally restricted |
1B
|
Expressed throughout most of the alar diencephalon and telencephalon, but was absent from their basal plate (summarized in Fig 1M).
|
Specimen
1C:
embryonic day 8.5
(more )
Note: rostral
Specimen
1C:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
rostral
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: future prosencephalon |
Present |
Regionally restricted |
1C
|
Expressed throughout most of the alar diencephalon and telencephalon, but was absent from their basal plate (summarized in Fig 1M).
|
Specimen
1G:
embryonic day 9.5
(more )
Note: caudal
Specimen
1G:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
caudal
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: diencephalon |
Strong |
Regionally restricted |
1G
|
Expressed throughout alar regions but not the basal plate (summarized in Fig 1N).
|
Specimen
1H:
embryonic day 9.5
(more )
Note: central
Specimen
1H:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
central
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: diencephalon |
Strong |
Regionally restricted |
1H
|
Expressed throughout alar regions but not the basal plate (summarized in Fig 1N).
|
TS15: telencephalon |
Strong |
Regionally restricted |
1H
|
Expressed throughout alar regions but not the basal plate (summarized in Fig 1N).
|
Specimen
1I:
embryonic day 9.5
(more )
Note: rostral
Specimen
1I:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
rostral
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: diencephalon |
Strong |
Regionally restricted |
1I
|
Expressed throughout alar regions but not the basal plate (summarized in Fig 1N).
|
TS15: telencephalon |
Strong |
Regionally restricted |
1I
|
Expressed throughout alar regions but not the basal plate (summarized in Fig 1N).
|
Specimen
2A:
embryonic day 10.5
(more )
Note: caudal
Specimen
2A:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
caudal
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: diencephalon |
Strong |
Regionally restricted |
2A
|
Expressed in the prethalamus, but was absent from the zona limitans intrathalamica (ZLI). In the thalamus, expression levels were graded from low near the ZLI to high at the boundary with the pretectum.
|
TS17: telencephalon |
Strong |
|
2A
|
|
TS17: forebrain-midbrain boundary region |
Strong |
Regionally restricted |
2A
|
Expressed in the pretectum.
|
Specimen
2B:
embryonic day 10.5
(more )
Note: central
Specimen
2B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
central
|
Specimen
2C:
embryonic day 10.5
(more )
Note: rostral
Specimen
2C:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
rostral
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: diencephalon |
Strong |
Regionally restricted |
2C
|
Expressed in the prethalamus, but was absent from the zona limitans intrathalamica (ZLI) and the mantle cells of the developing eminentia thalami. In the thalamus, expression levels were graded from low near the ZLI to high at the boundary with the pretectum.
|
TS17: telencephalon |
Strong |
|
2C
|
|
Specimen
2G:
embryonic day 11.5
(more )
Note: caudal
Specimen
2G:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
caudal
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: telencephalon |
Present |
|
2G
|
Many telencephalic progenitor cells express Pax6, they did not express Barhl2.
|
TS19: diencephalon |
Present |
Regionally restricted |
2G
|
Expressed in the region of progenitor cells (pTh-C domain) in the thalamic ventricular zone.
|
Specimen
2H:
embryonic day 11.5
(more )
Note: central
Specimen
2H:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
central
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: prethalamus |
Strong |
|
2H
|
|
TS19: diencephalon |
Present |
Regionally restricted |
2H
|
Expressed in the region of progenitor cells (pTh-C domain) in the thalamic ventricular zone. Expression remained low or absent in the narrow strip of progenitor cells (pTh-R) caudal to the zona limitans intrathalamica. No expression in zona limitans intrathalamica.
|
Specimen
2I:
embryonic day 11.5
(more )
Note: rostral
Specimen
2I:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
rostral
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: telencephalon mantle layer |
Present |
Regionally restricted |
2I
|
Differentiating cells in the mantle zone of the ventral telencephalon in certain regions had no expression.
|
TS19: diencephalon |
Present |
Regionally restricted |
2I
|
Expressed in the hypothalamus, which was complementary to the expression of Barhl2.
|
Specimen
3A:
embryonic day 12.5
(more )
Note: caudal
Specimen
3A:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
caudal
|
Specimen
3B:
embryonic day 12.5
(more )
Note: central
Specimen
3B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
central
|
Specimen
3C:
embryonic day 12.5
(more )
Note: rostral
Specimen
3C:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
rostral
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: prethalamus |
Present |
|
3C
|
Expressed in both progenitor and postmitotic zones.
|
TS20: pallium |
Present |
Regionally restricted |
3C
|
Expressed in the progenitor zone.
|
TS20: thalamus |
Present |
Regionally restricted |
3C
|
Expressed in the progenitor zone.
|
Specimen
3G:
embryonic day 13.5
(more )
Note: caudal
Specimen
3G:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
caudal
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: prethalamus |
Present |
|
3G
|
Expressed in both progenitor and postmitotic zones.
|
Specimen
3H:
embryonic day 13.5
(more )
Note: central
Specimen
3H:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
central
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: prethalamus |
Present |
|
3H
|
Expressed in both progenitor and postmitotic zones.
|
Specimen
3I:
embryonic day 13.5
(more )
Note: rostral
Specimen
3I:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
rostral
|
|