About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:314694 Douchi D, et al., Induction of Gastric Cancer by Successive Oncogenic Activation in the Corpus. Gastroenterology. 2021 Dec;161(6):1907-1923.e26
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7461884
Gene symbol: Pgc
Gene name: progastricsin (pepsinogen C)
Reporter: mCherry
Antibody: anti-RFP
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1B - lesser: postnatal adult; Pgctm1.1(cre/ERT2)Itoy/Pgc+ (more )
Note: Triple labeled: red - Pgc reporter; blue - Pgc protein (assay MGI:7463981); green - Cdh1 (assay MGI:7463981).
Structure Level Pattern Image Note
TS28: stomach lesser curvature Present Regionally restricted 1B left upper , 1B right upper Expression is seen in 56.9% of the corpus units in the lesser curvature.
Specimen 1B - greater: postnatal adult; Pgctm1.1(cre/ERT2)Itoy/Pgc+ (more )
Note: Triple labeled: red - Pgc reporter; blue - Pgc protein (assay MGI:7463981); green - Cdh1 (assay MGI:7463981).
Structure Level Pattern Image Note
TS28: stomach greater curvature Present Regionally restricted 1B right lower , 1B left lower Expression is seen in 60.7% of the corpus units in the lesser curvature.
Specimen 1F: postnatal adult; Pgctm1.1(cre/ERT2)Itoy/Pgc+ (more )
Note: Triple labeled: red - Pgc reporter; blue - Pgc protein (assay MGI:7463981); green - Mki67 (assay MGI:7463939).
Structure Cell Type Level Pattern Image Note
TS28: stomach glandular region gastric gland Present Non-Uniform 1F Merge , 1F upper inset , 1F Ki67 mCherry , 1F lower inset Nearly all expressing cells are found in the corpus base; only a few are found in the neck and isthmus.
TS28: stomach glandular region epithelium peptic cell Present 1F Merge , 1F Ki67 mCherry Expression is seen in chief cells.
Specimen 1H - H,K-ATPase: postnatal adult; Pgctm1.1(cre/ERT2)Itoy/Pgc+ (more )
Note: Double labeled: red - Pgc; green - Atp4a (assay MGI:7463974).
Structure Cell Type Level Pattern Image Note
TS28: stomach glandular region gastric gland Present 1H left , 1H left red inset , 1H left merge inset
TS28: stomach glandular region epithelium parietal cell Absent 1H left merge inset , 1H left , 1H left red inset There is no expression in parietal cells.
Specimen 1H - ChgA: postnatal adult; Pgctm1.1(cre/ERT2)Itoy/Pgc+ (more )
Note: Double labeled: red - Pgc; green - Chga (assay MGI:7463977).
Structure Cell Type Level Pattern Image Note
TS28: stomach glandular region gastric gland Present 1H center , 1H center merge inset , 1H center red inset
TS28: stomach glandular region epithelium enteroendocrine cell Absent 1H center red inset , 1H center merge inset , 1H center There is no expression in enteroendocrine cells.
Specimen 1H - Muc5ac: postnatal adult; Pgctm1.1(cre/ERT2)Itoy/Pgc+ (more )
Note: Double labeled: red - Pgc; green - Muc5ac (assay MGI:7463978).
Structure Cell Type Level Pattern Image Note
TS28: stomach glandular region gastric gland Present 1H right
TS28: stomach glandular region epithelium foveolar cell of stomach Absent 1H right There is no expression in pit cells
Specimen S2G: postnatal adult; Pgctm1.1(cre/ERT2)Itoy/Pgc+ (more )
Note: Triple labeled: red - Pgc reporter (mCherry); green - Pgc reporter (Cre; assay MGI:7461913); blue - Mki67 (assay MGI:7463939).
Structure Level Pattern Image Note
TS28: stomach glandular region gastric gland Present Non-Uniform S2G left , S2G center There is expression in the gland base of the corpus. Cre was expressed in most mCherry-expressing cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
12/10/2024
MGI 6.24 		The Jackson Laboratory