Antibody:	anti-CREB
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS24: lung	Present	Regionally restricted	3A	Expression in nuclei of most cells.
TS24: lung epithelium	Present	Regionally restricted	3A	Expression in epithelium from both proximal and distal lung.
TS24: lung mesenchyme	Present	Regionally restricted	3A	No expression in large number of mesenchymal cells, particularly surrounding large airways.

Structure	Level	Pattern	Image	Note
TS24: lung epithelium	Present		3A Proximal
TS24: lung mesenchyme	Present	Regionally restricted	3A Proximal	No expression in large number of mesenchymal cells, particularly surrounding large airways.

Structure	Level	Pattern	Image	Note
TS24: lung epithelium	Present		3A Distal

Structure	Level	Pattern	Image	Note
TS26: lung	Present	Regionally restricted	3B	Expression in nuclei of most cells.
TS26: lung epithelium	Present	Regionally restricted	3B	Expression in epithelium from both proximal and distal lung.
TS26: lung mesenchyme	Present	Regionally restricted	3B	No expression in large number of mesenchymal cells, particularly surrounding large airways.

Structure	Level	Pattern	Image	Note
TS26: lung epithelium	Present		3B Proximal
TS26: lung mesenchyme	Present		3B Proximal	No expression in large number of mesenchymal cells, particularly surrounding large airways.

Structure	Level	Pattern	Image	Note
TS26: lung epithelium	Present		3B Distal

Structure	Level	Pattern	Image	Note
TS26: lung	Strong		S1A	Expression strongly detected in wildtype lung.

Structure	Level	Pattern	Image	Note
TS26: lung	Absent		S1B	No expression could be seen in mutant.