Igs2tm1(Cdkn1a-cre/ERT2,-EGFP)Mxu
Targeted Allele Detail
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| Symbol: |
Igs2tm1(Cdkn1a-cre/ERT2,-EGFP)Mxu |
| Name: |
intergenic site 2; targeted mutation 1, Ming Xu |
| MGI ID: |
MGI:7469624 |
| Synonyms: |
p21-Cre |
| Gene: |
Igs2 Location: unknown Genetic Position: Chr11, Syntenic
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| Alliance: |
Igs2tm1(Cdkn1a-cre/ERT2,-EGFP)Mxu page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:335288
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| Parent Cell Line: |
Not Specified (ES Cell)
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| Strain of Origin: |
Not Specified
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| Allele Type: |
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Targeted (Inducible, Recombinase, Reporter) |
| Inducer: |
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tamoxifen |
| Mutation: |
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Insertion
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Igs2tm1(Cdkn1a-cre/ERT2,-EGFP)Mxu expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Cdkn1a (MGI:104556) |
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Mutation details: Site-specific PhiC31 integrase-mediated transgenesis is used to insert a 3,225-bp mouse Cdkn1a (p21) promoter fragment followed by a bicistronic message consisting of the coding sequence for Cre fused to an ERT2 domain, an IRES followed by an ORF coding for enhanced GFP into the intergenic site 2 locus (Hipp11). This construct was microinjected into the pronucleus of recipient zygotes containing attP sites in the Hipp11 locus. Site-specific recombination occurred between the attB sites in the plasmid and attP sites in the H11 genomic locus. Positive embryonic stem cell clones were then implanted into C57BL/6 females.
(J:335288)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Cdkn1a
(mouse)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Igs2 Mutation: |
72 strains or lines available
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| Original: |
J:335288 Wang B, et al., An inducible p21-Cre mouse model to monitor and manipulate p21-highly-expressing senescent cells in vivo. Nat Aging. 2021 Oct;1(10):962-973 |
| All: |
3 reference(s) |
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Analysis Tools
