Gene Expression Data
Assay Details
Assay
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Reference:
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J:324832
Sun Y, et al., Single-cell transcriptomic landscapes of the otic neuronal lineage at multiple early embryonic ages. Cell Rep. 2022 Mar 22;38(12):110542
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:7493888
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Gene symbol:
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Casz1
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Gene name:
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castor zinc finger 1
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Reporter:
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HA
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Antibody:
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anti-HA tag
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Detection system:
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Secondary antibody
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Results
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Specimen
S7G:
embryonic day 13.5; Casz1em1Zyliu/Casz1 +
(more )
Note: Double labeled: green - Casz1; blue - Hoechst.
Specimen
S7G:
(close )
Genetic Background:
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C57BL/6J-Casz1em1Zyliu/J
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Mutant Allele(s):
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Casz1em1Zyliu/Casz1+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Casz1; blue - Hoechst.
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS22: cochlear ganglion |
neuron |
Present |
|
S7G'
,
S7G
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Expression is significantly higher in vestibular ganglionic cells than spiral ones.
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TS22: vestibular ganglion |
neuron |
Present |
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S7G'
,
S7G
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Expression is significantly higher in vestibular ganglionic cells than spiral ones.
|
Specimen
S7H:
embryonic day 13.5; Casz1em1Zyliu/Casz1 +
(more )
Note: Double labeled: green - Casz1; blue - Hoechst.
Specimen
S7H:
(close )
Genetic Background:
|
C57BL/6J-Casz1em1Zyliu/J
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Mutant Allele(s):
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Casz1em1Zyliu/Casz1+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Note:
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Double labeled: green - Casz1; blue - Hoechst.
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Specimen
S7I:
embryonic day 13.5; Casz1em1Zyliu/Casz1 +
(more )
Note: Triple labeled: green - Casz1 reporter; red - Myo6 (assay MGI:7493892); blue - Hoechst.
Specimen
S7I:
(close )
Genetic Background:
|
C57BL/6J-Casz1em1Zyliu/J
|
Mutant Allele(s):
|
Casz1em1Zyliu/Casz1+
|
Age:
|
embryonic day 13.5
|
Sex:
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Not Specified
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Type:
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section
|
Fixation:
|
4% Paraformaldehyde
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Note:
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Triple labeled: green - Casz1 reporter; red - Myo6 (assay MGI:7493892); blue - Hoechst.
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Specimen
S7K:
postnatal day 3; Casz1em1Zyliu/Casz1 +
(more )
Note: Double labeled: green - Casz1; blue - Hoechst.
Specimen
S7K:
(close )
Genetic Background:
|
C57BL/6J-Casz1em1Zyliu/J
|
Mutant Allele(s):
|
Casz1em1Zyliu/Casz1+
|
Age:
|
postnatal day 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Note:
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Double labeled: green - Casz1; blue - Hoechst.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: cochlear ganglion |
spiral ganglion neuron |
Present |
|
S7K
|
Expression is significantly higher in vestibular ganglionic cells than spiral ones.
|
TS27: vestibular ganglion |
neuron |
Present |
|
S7K
|
Expression is significantly higher in vestibular ganglionic cells than spiral ones.
|
Specimen
S7K inset:
postnatal day 3; Casz1em1Zyliu/Casz1 +
(more )
Note: Triple labeled: green - Casz1 reporter; red - Map2 (assay MGI:7493890); blue - Hoechst.
Specimen
S7K inset:
(close )
Genetic Background:
|
C57BL/6J-Casz1em1Zyliu/J
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Mutant Allele(s):
|
Casz1em1Zyliu/Casz1+
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Age:
|
postnatal day 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Note:
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Triple labeled: green - Casz1 reporter; red - Map2 (assay MGI:7493890); blue - Hoechst.
|
Specimen
S7L:
postnatal day 10; Casz1em1Zyliu/Casz1 +
(more )
Note: Triple labeled: green - Casz1 reporter; red - Map2 (assay MGI:7493890); blue - Hoechst.
Specimen
S7L:
(close )
Genetic Background:
|
C57BL/6J-Casz1em1Zyliu/J
|
Mutant Allele(s):
|
Casz1em1Zyliu/Casz1+
|
Age:
|
postnatal day 10
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
Triple labeled: green - Casz1 reporter; red - Map2 (assay MGI:7493890); blue - Hoechst.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: cochlear ganglion |
spiral ganglion neuron |
Present |
Regionally restricted |
S7L
,
S7L'
|
Expression is seen in some, but not all, neurons.
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Specimen
S7M:
postnatal day 10; Casz1em1Zyliu/Casz1 +
(more )
Note: Triple labeled: green - Casz1 reporter; red - Map2 (assay MGI:7493890); blue - Hoechst.
Specimen
S7M:
(close )
Genetic Background:
|
C57BL/6J-Casz1em1Zyliu/J
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Mutant Allele(s):
|
Casz1em1Zyliu/Casz1+
|
Age:
|
postnatal day 10
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
Triple labeled: green - Casz1 reporter; red - Map2 (assay MGI:7493890); blue - Hoechst.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: vestibular ganglion |
neuron |
Present |
Regionally restricted |
S7M'
,
S7M
|
Expression is seen in some, but not all, neurons.
|
Specimen
S7N:
postnatal day 21; Casz1em1Zyliu/Casz1 +
(more )
Note: Triple labeled: green - Casz1 reporter; red - Map2 (assay MGI:7493890); blue - Hoechst.
Specimen
S7N:
(close )
Genetic Background:
|
C57BL/6J-Casz1em1Zyliu/J
|
Mutant Allele(s):
|
Casz1em1Zyliu/Casz1+
|
Age:
|
postnatal day 21
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Sex:
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Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Triple labeled: green - Casz1 reporter; red - Map2 (assay MGI:7493890); blue - Hoechst.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: cochlear ganglion |
spiral ganglion neuron |
Present |
Regionally restricted |
S7N'
,
S7N
|
Expression is seen in some, but not all, neurons.
|
Specimen
S7O:
postnatal day 21; Casz1em1Zyliu/Casz1 +
(more )
Note: Triple labeled: green - Casz1 reporter; red - Map2 (assay MGI:7493890); blue - Hoechst.
Specimen
S7O:
(close )
Genetic Background:
|
C57BL/6J-Casz1em1Zyliu/J
|
Mutant Allele(s):
|
Casz1em1Zyliu/Casz1+
|
Age:
|
postnatal day 21
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Sex:
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Not Specified
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Type:
|
section
|
Fixation:
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4% Paraformaldehyde
|
Note:
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Triple labeled: green - Casz1 reporter; red - Map2 (assay MGI:7493890); blue - Hoechst.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: vestibular ganglion |
neuron |
Present |
Regionally restricted |
S7O'
,
S7O
|
Expression is seen in some, but not all, neurons.
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|