Slc30a3tm1.1(cre/ERT2)Mlds
Targeted Allele Detail
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| Symbol: |
Slc30a3tm1.1(cre/ERT2)Mlds |
| Name: |
solute carrier family 30 (zinc transporter), member 3; targeted mutation 1.1, Michael Michaelides |
| MGI ID: |
MGI:7512765 |
| Synonyms: |
ZnT3-CreERT2/Rox-mCherry |
| Gene: |
Slc30a3 Location: Chr5:31243450-31265576 bp, - strand Genetic Position: Chr5, 16.97 cM
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| Alliance: |
Slc30a3tm1.1(cre/ERT2)Mlds page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:338408
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| Parent Cell Line: |
iTL IC1 (ES Cell)
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| Strain of Origin: |
C57BL/6NTac
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| Allele Type: |
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Targeted (Conditional ready, Inducible, Recombinase, Reporter) |
| Inducer: |
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tamoxifen |
| Mutation: |
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Insertion
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Slc30a3tm1.1(cre/ERT2)Mlds expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: The targeting constructs consist of the 3 long homology arm (LA) extends ~6.6 kb 3 to the Rox12/Rox flanked mCherry inversion cassette, and the 5 short homology arm (SA) extends about 3 kb 5 to the 5 Cre/ERT sequence. Homologous recombination in the ES cells inserted neo selection cassette, cre/ERT2 and Rox flanked mCherry into the Slc30a3 gene. The 5 Cre is in-frame fused to the endogenous ATG start site. The 3 CreERT2 is fused with P2A-exon 1 coding sequence. The mCherry inversion cassette is inserted in intron 2. Exon 2 and the inversion cassette are flanked by ROX12/ROX sites for conditionally expressing mCherry reporter. The neo-selection cassette was removed by crossing the resulting chimeric mice to flippase (FLP) deleter mice.
(J:338408)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Slc30a3
(mouse)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Slc30a3 Mutation: |
28 strains or lines available
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| Original: |
J:338408 Kouvaros S, et al., A CRE/DRE dual recombinase transgenic mouse reveals synaptic zinc-mediated thalamocortical neuromodulation. Sci Adv. 2023 Jun 9;9(23):eadf3525 |
| All: |
1 reference(s) |
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Analysis Tools
