Cdkn2aem1(flpo,HBEGF/mCherry)Jpmb
Endonuclease-mediated Allele Detail
|
|
| Symbol: |
Cdkn2aem1(flpo,HBEGF/mCherry)Jpmb |
| Name: |
cyclin dependent kinase inhibitor 2A; endonuclease-mediated mutation 1, Juan Pedro Martinez-Barbera |
| MGI ID: |
MGI:7514135 |
| Synonyms: |
p16-FDR |
| Gene: |
Cdkn2a Location: Chr4:89192710-89212856 bp, - strand Genetic Position: Chr4, 42.15 cM, cytoband C3-C6
|
| Alliance: |
Cdkn2aem1(flpo,HBEGF/mCherry)Jpmb page
|
|
| Germline Transmission: |
Earliest citation of germline transmission:
J:338213
|
| Parent Cell Line: |
CCE/EK.CCE (ES Cell)
|
| Strain of Origin: |
129S/SvEv-Gpi1c
|
|
| Allele Type: |
|
Endonuclease-mediated (Inserted expressed sequence, Recombinase, Reporter) |
| Mutation: |
|
Insertion
|
| |
|
Cdkn2aem1(flpo,HBEGF/mCherry)Jpmb expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Cdkn2a (MGI:104738) |
|
|
| |
|
Mutation details: Using CRISPR/Cas9 system, a bicistronic targeting vector containing a mammalian optimized flippase (FLP) recombinase along with a diphtheria toxin receptor (HBEGF) fused with a mCherry fluorescent reporter separated by P2A sequences was inserted after the Stop codon in the exon3 of the Cdkn2a in ES cells.
(J:338213)
|
|
|
|
Activity: |
 Tissue activity of this recombinase allele
|
|
Driver:
|
Cdkn2a
(mouse)
|
| |
|
|
| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
| Carrying any Cdkn2a Mutation: |
64 strains or lines available
|
|
| Original: |
J:338213 Haston S, et al., Clearance of senescent macrophages ameliorates tumorigenesis in KRAS-driven lung cancer. Cancer Cell. 2023 Jul 10;41(7):1242-1260.e6 |
| All: |
1 reference(s) |
|
Analysis Tools
