About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:262706 Miao YL, et al., Mediator complex component MED13 regulates zygotic genome activation and is required for postimplantation development in the mouse. Biol Reprod. 2018 Apr 1;98(4):449-464
Assay type: RT-PCR
MGI Accession ID: MGI:7520147
Gene symbol: Med13
Gene name: mediator complex subunit 13
Probe: Med13-pF1, Med13-pR1
Assay notes: Quantitative RT-PCR. Relative expression was normalized to E2f. Two Med13 primer sets were described, but the one used in this assay was not detailed. Primer set Med13-pF2, Med13-pR2 MGI:7520140 may have been used in this assay.
Results Image: 1A
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
ooc P adult TS28: primary oocyte Present Not Specified; total RNA Not Specified Female
egg P adult TS28: secondary oocyte Present Not Specified; total RNA Not Specified Female
1C E0.5 (a) TS1: 1-cell stage embryo Present Not Specified; total RNA Not Specified Not Specified
2C E1.0 (a) TS2: 2-cell stage embryo Present (b) Not Specified; total RNA Not Specified Not Specified
Notes:
(a) Age assigned by curator based on morphological criteria supplied by authors.
(b) Expression remained relatively stable through the 2-cell stage.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
09/17/2024
MGI 6.24 		The Jackson Laboratory