Pcnt Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:263975 Xie Z, et al., A Golgi Lipid Signaling Pathway Controls Apical Golgi Distribution and Cell Polarity during Neurogenesis. Dev Cell. 2018 Mar 26;44(6):725-740.e4
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7520526
Gene symbol:	Pcnt
Gene name:	pericentrin (kendrin)

Antibody:	Anti-Pericentrin (clone 30)
Detection system:	Biotinylated secondary antibody/[Strept]avidin

Results

Specimen S3E Pcnt Control: embryonic day 12.5; Emx1^tm1(cre)Krj/?, Pitpna^tm2.1Vab/?, Pitpnb^tm2.1Vab/? (more

)
Note: Conditional mutant. Littermate embryos with at least one non-floxed allele of Pitpna or Pitpnb (i.e. Pitpnafl/+ Pitpnbfl/fl Emx1Cre/+, Pitpnafl/+ Pitpnbfl/fl Emx1Cre/Cre, Pitpnafl/fl Pitpnbfl/+ Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/+ Emx1Cre/Cre) were used as controls. Double labeled: red - Pcnt; blue - Pard3 (assay MGI:7520523).

Structure	Level	Pattern	Image	Note
TS20: neocortex	Present		S3E Control Pericentrin , S3E Control Merge

Specimen S3E Pcnt DKO: embryonic day 12.5; Emx1^tm1(cre)Krj/?, Pitpna^tm2.1Vab/Pitpna^tm2.1Vab, Pitpnb^tm2.1Vab/Pitpnb^tm2.1Vab (more

)
Note: Conditional mutant. Tissue-specific double knockout progeny (designated DKO) were either Pitpnafl/fl Pitpnbfl/fl Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/fl Emx1Cre/Cre. Double labeled: red - Pcnt; blue - Pard3 (assay MGI:7520523).

Structure	Level	Pattern	Image	Note
TS20: neocortex	Present	Regionally restricted	S3E DKO Pericentrin , S3E DKO Merge	Localization of centrosome to ventricular surface similar to control.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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