Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:215514
Saulnier A, et al., The doublesex homolog Dmrt5 is required for the development of the caudomedial cerebral cortex in mammals. Cereb Cortex. 2013 Nov;23(11):2552-67
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:7522038
|
Gene symbol:
|
Etv1
|
Gene name:
|
ets variant 1
|
Probe:
|
Er81 probe
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Alkaline phosphatase
|
|
Results
|
Specimen
6K Dmrt5+/+:
embryonic day 12.5
(more )
Specimen
6K Dmrt5<+/+>:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cortical plate |
Present |
Graded |
6K Dmrt5+/+
|
Expression was detected in a high-ventral to low-dorsal gradient.
|
Specimen
6K Dmrt5<-/->:
(close )
Genetic Background:
|
involves: C57BL/6
|
Mutant Allele(s):
|
Dmrta2tm1Ejbd/Dmrta2tm1Ejbd
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: telencephalon |
Present |
Regionally restricted |
6K Dmrt5-/-
|
Expression was detected more medially in the lateral wall of the telencephalon.
|
Specimen
S10B Dmrt5+/+:
embryonic day 18.5
(more )
Specimen
S10B Dmrt5<+/+>:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: neocortex |
Present |
Regionally restricted |
S10B Er81 Dmrt5+/+
|
Layer V marker.
|
Specimen
S10B Dmrt5<-/->:
(close )
Genetic Background:
|
involves: C57BL/6
|
Mutant Allele(s):
|
Dmrta2tm1Ejbd/Dmrta2tm1Ejbd
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: neocortex |
Present |
Regionally restricted |
S10B Er81 Dmrt5-/-
|
In the mutant, expression appeared in a pial to ventricular order similar to wild type.
|
|