Gene Expression Data
Assay Details
Assay
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Reference:
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J:268103
Dykes IM, et al., A Requirement for Zic2 in the Regulation of Nodal Expression Underlies the Establishment of Left-Sided Identity. Sci Rep. 2018 Jul 11;8(1):10439
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7530986
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Gene symbol:
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Dand5
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Gene name:
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DAN domain family member 5, BMP antagonist
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Probe:
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cerl-2 probe1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
4o:
embryonic day 8.0
(more )
Note: 3-somite stage
Specimen
4o:
(close )
Genetic Background:
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involves: C3H/HeH * C57BL/6
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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3-somite stage
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Structure |
Level |
Pattern |
Image |
Note |
TS12: node |
Present |
Non-Uniform |
4o
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Asymmetrically expressed at the node (L < R).
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Specimen
4q:
embryonic day 8.0; Zic2iso/ Zic2iso
(more )
Note: 3-somite stage
Specimen
4q:
(close )
Genetic Background:
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involves: C3H/HeH * C57BL/6
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Mutant Allele(s):
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Zic2iso/Zic2iso
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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3-somite stage
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Structure |
Level |
Pattern |
Image |
Note |
TS12: node |
Present |
Non-Uniform |
4q
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Asymmetrically expressed at the node (L < R), similar as in the wild-type.
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Specimen
4p:
embryonic day 8.0
(more )
Note: 2-somite stage
Specimen
4p:
(close )
Genetic Background:
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involves: C3H/HeH * C57BL/6
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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2-somite stage
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Structure |
Level |
Pattern |
Image |
Note |
TS12: node |
Present |
Non-Uniform |
4p
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Asymmetrically expressed at the node (L < R).
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Specimen
4r:
embryonic day 8.0; Zic2iso/ Zic2iso
(more )
Note: 2-somite stage
Specimen
4r:
(close )
Genetic Background:
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involves: C3H/HeH * C57BL/6
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Mutant Allele(s):
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Zic2iso/Zic2iso
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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2-somite stage
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Structure |
Level |
Pattern |
Image |
Note |
TS12: node |
Present |
Non-Uniform |
4r
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Asymmetrically expressed at the node (L > R), which was different from the wild-type.
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Specimen
not shown WT/Het:
embryonic day 8.0
(more )
Note: headfold and 1-4 somites, summarized in Fig 4s
Specimen
not shown WT/Het:
(close )
Genetic Background:
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involves: C3H/HeH * C57BL/6
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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headfold and 1-4 somites, summarized in Fig 4s
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Structure |
Level |
Pattern |
Image |
Note |
TS12: node |
Present |
|
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Enriched on the right side of the node in 8 of 9 embryos: strong expression in 7 embryos, weak in 1. Symmetrically expressed (strong, L=R) in 1 out of 9 embryos.
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Specimen
not shown iso/iso:
embryonic day 8.0; Zic2iso/ Zic2iso
(more )
Note: headfold and 1-4 somites, summarized in Fig 4s
Specimen
not shown iso/iso:
(close )
Genetic Background:
|
involves: C3H/HeH * C57BL/6
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Mutant Allele(s):
|
Zic2iso/Zic2iso
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Age:
|
embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
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Note:
|
headfold and 1-4 somites, summarized in Fig 4s
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Structure |
Level |
Pattern |
Image |
Note |
TS12: node |
Present |
|
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Expression was maintained at wild-type levels (strong expression in 4 embryos, weak in 2), but the onset of its laterality appeared to be delayed and slightly perturbed: either L>R (in 2 embryos), L=R (in 2 embryos), or L<R (in 2 embryos).
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