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Gene Expression Data
Assay Details
Assay
Reference: J:293794 Durbin MD, et al., SHROOM3 is downstream of the planar cell polarity pathway and loss-of-function results in congenital heart defects. Dev Biol. 2020 Aug 15;464(2):124-136
Assay type: Western blot
MGI Accession ID: MGI:7531401
Gene symbol: Ppp1r12a
Gene name: protein phosphatase 1, regulatory subunit 12A
Antibody: anti-phospho-MYPT1 (Thr8530) (PA5-38299)
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: The antibody used in this assay recognizes the activated form of the protein.
Results
Image: 5X pMYPT ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Genetic BackgroundMutant Allele(s)Sex Note
Shroom3+/+ E12.5,14.5 TS20: heart
TS22: heart 		Present C57BL/6J Not Specified It was unclear whether the pooled hearts were of both ages or if each age was examined individually.
Shroom3+/+ E12.5,14.5 TS20: heart
TS22: heart 		Present C57BL/6J Not Specified It was unclear whether the pooled hearts were of both ages or if each age was examined individually.
Shroom3+/+ E12.5,14.5 TS20: heart
TS22: heart 		Present C57BL/6J Not Specified It was unclear whether the pooled hearts were of both ages or if each age was examined individually.
Shroom3gt/gt E12.5,14.5 TS20: heart
TS22: heart 		Present (a) B6.129S4-Shroom3Gt(ROSA53)Sor/J Shroom3Gt(ROSA53)Sor/Shroom3Gt(ROSA53)SorNot Specified It was unclear whether the pooled hearts were of both ages or if each age was examined individually.
Shroom3gt/gt E12.5,14.5 TS20: heart
TS22: heart 		Present (a) B6.129S4-Shroom3Gt(ROSA53)Sor/J Shroom3Gt(ROSA53)Sor/Shroom3Gt(ROSA53)SorNot Specified It was unclear whether the pooled hearts were of both ages or if each age was examined individually.
Shroom3gt/gt E12.5,14.5 TS20: heart
TS22: heart 		Present (a) B6.129S4-Shroom3Gt(ROSA53)Sor/J Shroom3Gt(ROSA53)Sor/Shroom3Gt(ROSA53)SorNot Specified It was unclear whether the pooled hearts were of both ages or if each age was examined individually.
Notes:
(a) Expression levels are reduced in the mutant relative to those seen in wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/10/2024
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