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Gene Expression Data
Assay Details
Assay
Reference: J:321979 Kurz J, et al., Notch signaling is a novel regulator of visceral smooth muscle cell differentiation in the murine ureter. Development. 2022 Feb 15;149(4):dev199735
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7531485
Gene symbol: Jag1
Gene name: jagged 1
Antibody: anti-Jag1 (H-114; sc-8303)
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: This is an in situ proximity ligation assay (PLA) assay using the Duolink In Situ Red Starter Kit Mouse/Rabbit system.
Results
Specimen 1B E12.5 with Notch1: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: epithelial layer of ureter Present Regionally restricted 1B E12.5 Notch1/Jag1 Proximity signals were observed for JAG1 with NOTCH1 in the ureteric mesenchyme and ureteric epithelium.
TS20: ureteral mesenchyme Present Regionally restricted 1B E12.5 Notch1/Jag1 Proximity signals were observed for JAG1 with NOTCH1 in the ureteric mesenchyme and ureteric epithelium.
Specimen 1B E14.5 with Notch1: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: ureteral mesenchyme Present 1B E14.5 Notch1/Jag1 Proximity signals were observed for JAG1 with NOTCH1 in the ureteric mesenchyme and ureteric epithelium.
TS22: urothelium of ureter Present 1B E14.5 Notch1/Jag1 Proximity signals were observed for JAG1 with NOTCH1 in the ureteric mesenchyme and ureteric epithelium.
Specimen 1B E16.5 with Notch1: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: ureter Ambiguous 1B E16.5 Notch1/Jag1 At E16.5, proximity signals were not observed.
Specimen 1B E18.5 with Notch1: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: ureteral mesenchyme Present 1B E18.5 Notch1/Jag1 Proximity signals were observed for JAG1 with NOTCH1 in the ureteric mesenchyme and ureteric epithelium.
TS26: urothelium of ureter Present 1B E18.5 Notch1/Jag1 Proximity signals were observed for JAG1 with NOTCH1 in the ureteric mesenchyme and ureteric epithelium.
Specimen 1B E16.5 dorsal aorta with Notch1: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: descending aorta Ambiguous 1B dorsal aorta Notch1/Jag1
Specimen 1B E12.5 with Notch2: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: epithelial layer of ureter Present 1B E12.5 Notch2/Jag1 Proximity signals were observed for JAG1 with NOTCH2 in the ureteric mesenchyme and ureteric epithelium.
TS20: ureteral mesenchyme Present 1B E12.5 Notch2/Jag1 Proximity signals were observed for JAG1 with NOTCH2 in the ureteric mesenchyme and ureteric epithelium.
Specimen 1B E14.5 with Notch2: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: ureteral mesenchyme Present 1B E14.5 Notch2/Jag1 Proximity signals were observed for JAG1 with NOTCH2 in the ureteric mesenchyme and ureteric epithelium.
TS22: urothelium of ureter Present 1B E14.5 Notch2/Jag1 Proximity signals were observed for JAG1 with NOTCH2 in the ureteric mesenchyme and ureteric epithelium.
Specimen 1B E16.5 with Notch2: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: ureter Ambiguous 1B E16.5 Notch2/Jag1 At E16.5, proximity signals were not observed.
Specimen 1B E18.5 with Notch2: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: ureteral mesenchyme Present 1B E18.5 Notch2/Jag1 Proximity signals were observed for JAG1 with NOTCH2 in the ureteric mesenchyme and ureteric epithelium.
TS26: urothelium of ureter Present 1B E18.5 Notch2/Jag1 Proximity signals were observed for JAG1 with NOTCH2 in the ureteric mesenchyme and ureteric epithelium.
Specimen 1B E16.5 dorsal aorta with Notch2: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: descending aorta Strong 1B dorsal aorta Notch2/Jag1 Strong label was noted with Jag1 and Notch2 in perivascular cells.
Specimen 1B E12.5 with Notch3: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: epithelial layer of ureter Present 1B E12.5 Notch3/Jag1 Proximity signals were observed for JAG1 with NOTCH3 in the ureteric mesenchyme and ureteric epithelium.
TS20: ureteral mesenchyme Present 1B E12.5 Notch3/Jag1 Proximity signals were observed for JAG1 with NOTCH3 in the ureteric mesenchyme and ureteric epithelium.
Specimen 1B E14.5 with Notch3: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: ureteral mesenchyme Present 1B E14.5 Notch3/Jag1 Proximity signals were observed for JAG1 with NOTCH3 in the ureteric mesenchyme and ureteric epithelium.
TS22: urothelium of ureter Present 1B E14.5 Notch3/Jag1 Proximity signals were observed for JAG1 with NOTCH3 in the ureteric mesenchyme and ureteric epithelium.
Specimen 1B E16.5 with Notch3: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: ureter Ambiguous 1B E16.5 Notch3/Jag1 At E16.5, proximity signals were not observed.
Specimen 1B E18.5 with Notch3: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: ureteral mesenchyme Present 1B E18.5 Notch3/Jag1 Proximity signals were observed for JAG1 with NOTCH3 in the ureteric mesenchyme and ureteric epithelium.
TS26: urothelium of ureter Present 1B E18.5 Notch3/Jag1 Proximity signals were observed for JAG1 with NOTCH3 in the ureteric mesenchyme and ureteric epithelium.
Specimen 1B E16.5 dorsal aorta with Notch3: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: descending aorta Present 1B dorsal aorta Notch3/Jag1 Strong label was noted with Jag1 and Notch3 in perivascular cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/17/2024
MGI 6.24 		The Jackson Laboratory