Gene Expression Data
Assay Details
Assay
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Reference:
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J:336094
Zheng H, et al., CHIP inhibits odontoblast differentiation through promoting DLX3 polyubiquitylation and degradation. Development. 2023 May 15;150(10):dev200848
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7532783
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Gene symbol:
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Stub1
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Gene name:
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STIP1 homology and U-Box containing protein 1
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Antibody:
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Anti-Stub1 (ab134064) [EPR4447]
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Detection system:
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Secondary antibody
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Assay notes:
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An in situ proximity ligation assay (PLA) using Stub1 (CHIP) and DLX3 antibodies.
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Results
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Specimen
5C left:
postnatal day 1
(more )
Note: Red fluorescent signals represent the presence of protein interaction.
Specimen
5C left:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Red fluorescent signals represent the presence of protein interaction.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: molar mesenchyme |
mesenchymal cell |
Present |
Regionally restricted |
5C left
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Proximity ligation assay indicated that Stub1 interacted with Dlx3 in undifferentiated dental mesenchymal cells.
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Specimen
5C right:
postnatal day 1
(more )
Note: Red fluorescent signals represent the presence of protein interaction.
Specimen
5C right:
(close )
Genetic Background:
|
Not Specified
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Red fluorescent signals represent the presence of protein interaction.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: molar |
preodontoblast |
Present |
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5C right
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Proximity ligation assay indicated that Stub1 interacted with Dlx3 in preodontoblasts of P1 molars.
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Specimen
7Fa:
postnatal day 14
(more )
Note: Red fluorescent signals represent the presence of protein interaction.
Specimen
7Fa:
(close )
Genetic Background:
|
Not Specified
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Age:
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postnatal day 14
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Red fluorescent signals represent the presence of protein interaction.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: incisor mesenchyme |
mesenchymal cell |
Present |
Regionally restricted |
7Fa
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Proximity ligation assay indicated that Stub1 interacted with Dlx3 in undifferentiated dental mesenchymal cells.
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Specimen
7Fb:
postnatal day 14
(more )
Note: Red fluorescent signals represent the presence of protein interaction.
Specimen
7Fb:
(close )
Genetic Background:
|
Not Specified
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Age:
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postnatal day 14
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Paraffin
|
Note:
|
Red fluorescent signals represent the presence of protein interaction.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: incisor |
odontoblast |
Weak |
Regionally restricted |
7Fb
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Proximity ligation assay indicated that Stub1-Dlx3 complexes were decreased in secretory odontoblasts.
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