Gene Expression Data
Assay Details
Assay
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Reference:
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J:296266
Ogino H, et al., The Secreted Glycoprotein Reelin Suppresses the Proliferation and Regulates the Distribution of Oligodendrocyte Progenitor Cells in the Embryonic Neocortex. J Neurosci. 2020 Sep 30;40(40):7625-7636
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7539228
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Gene symbol:
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Pdgfra
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Gene name:
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platelet derived growth factor receptor, alpha polypeptide
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Results
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Specimen
1D +/+:
embryonic day 18.5
(more )
Note: Triple labeled: red - Pdgfra; blue - Olig2 (assay MGI:7539229); green - Vldlr (assay MGI:7539224).
Specimen
1D +/+:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Pdgfra; blue - Olig2 (assay MGI:7539229); green - Vldlr (assay MGI:7539224).
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Specimen
1D -/-:
(close )
Genetic Background:
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involves: 129S7/SvEvBrd * Slc:ICR
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Mutant Allele(s):
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Vldlrtm1Her/Vldlrtm1Her
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Pdgfra; blue - Olig2 (assay MGI:7539229); green - Vldlr (assay MGI:7539224).
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Specimen
4E Reln+/+:
embryonic day 18.5
(more )
Note: Double labeled: red - Pdgfra; green - Mki67 (assay MGI:7539232).
Specimen
4E Reln+/+:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pdgfra; green - Mki67 (assay MGI:7539232).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: parietal cortex |
oligodendrocyte precursor cell |
Present |
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4E +/+
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Primary somatosensory cortex was shown.
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Specimen
4E Reln+/-:
embryonic day 18.5; Relnrl/Reln +
(more )
Note: Double labeled: red - Pdgfra; green - Mki67 (assay MGI:7539232).
Specimen
4E Reln+/-:
(close )
Genetic Background:
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B6C3Fe a/a-Relnrl/J
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Mutant Allele(s):
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Relnrl/Reln+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pdgfra; green - Mki67 (assay MGI:7539232).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: parietal cortex |
oligodendrocyte precursor cell |
Present |
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4E +/-
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Primary somatosensory cortex was shown. Dramatically increased density (approximately a 50% increase), and the distribution markedly shifted toward the superficial layer. Immunofluorescent signal was also much stronger compared with the control.
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Specimen
4J Dab1+/+:
embryonic day 18.5
(more )
Note: Double labeled: red - Pdgfra; green - Mki67 (assay MGI:7539232).
Specimen
4J Dab1+/+:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pdgfra; green - Mki67 (assay MGI:7539232).
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: parietal cortex |
oligodendrocyte precursor cell |
Present |
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4J left
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Primary somatosensory cortex was shown.
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Specimen
4J Dab1+/yot:
embryonic day 18.5; Dab1yot/Dab1 +
(more )
Note: Double labeled: red - Pdgfra; green - Mki67 (assay MGI:7539232).
Specimen
4J Dab1+/yot:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Dab1yot/Dab1+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pdgfra; green - Mki67 (assay MGI:7539232).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: parietal cortex |
oligodendrocyte precursor cell |
Present |
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4J right
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Primary somatosensory cortex was shown. Dramatically increased density (approximately a 50% increase), and the distribution markedly shifted toward the superficial layer. Immunofluorescent signal was also much stronger compared with the control.
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Specimen
5D Vldlr+/+:
embryonic day 18.5
(more )
Note: Double labeled: red - Pdgfra; green - Mki67 (assay MGI:7539232).
Specimen
5D Vldlr+/+:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pdgfra; green - Mki67 (assay MGI:7539232).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: parietal cortex |
oligodendrocyte precursor cell |
Present |
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5D +/+
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Primary somatosensory cortex was shown.
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Specimen
5D Vldlr-/-:
(close )
Genetic Background:
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involves: 129S7/SvEvBrd * Slc:ICR
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Mutant Allele(s):
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Vldlrtm1Her/Vldlrtm1Her
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pdgfra; green - Mki67 (assay MGI:7539232).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: parietal cortex |
oligodendrocyte precursor cell |
Present |
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5D -/-
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Primary somatosensory cortex was shown. Increased density by around 50%.
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Specimen
6D Adamts3+/+:
embryonic day 18.5
(more )
Note: Double labeled: red - Pdgfra; green - Mki67 (assay MGI:7539232).
Specimen
6D Adamts3+/+:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pdgfra; green - Mki67 (assay MGI:7539232).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: parietal cortex |
oligodendrocyte precursor cell |
Present |
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6D +/+
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Primary somatosensory cortex was shown.
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Specimen
6D Adamts3-/-:
(close )
Genetic Background:
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C57BL/6N-Adamts3tm1b(KOMP)Wtsi/Wtsi
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Mutant Allele(s):
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Adamts3tm1b(KOMP)Wtsi/Adamts3tm1b(KOMP)Wtsi
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pdgfra; green - Mki67 (assay MGI:7539232).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: parietal cortex |
oligodendrocyte precursor cell |
Present |
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6D -/-
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Primary somatosensory cortex was shown. The number of positive cells was significantly decreased compared with the control. Immunofluorescent signal was markedly weaker compared with the control.
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Specimen
6I Reln+/+:
embryonic day 18.5
(more )
Note: Double labeled: red - Pdgfra; green - Mki67 (assay MGI:7539232).
Specimen
6I Reln+/+:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pdgfra; green - Mki67 (assay MGI:7539232).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: parietal cortex |
oligodendrocyte precursor cell |
Present |
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6I left
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Primary somatosensory cortex was shown.
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Specimen
6I Reln PA-DV/PA-DV:
(close )
Genetic Background:
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involves: C57BL/6J * C57BL/6N
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Mutant Allele(s):
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Relnem1Mhat/Relnem1Mhat
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pdgfra; green - Mki67 (assay MGI:7539232).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: parietal cortex |
oligodendrocyte precursor cell |
Present |
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6I right
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Primary somatosensory cortex was shown. The number of positive cells was significantly decreased compared with the control. Immunofluorescent signal was markedly weaker compared with the control.
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