Ightm3.1(icre)Mbu
Targeted Allele Detail
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| Symbol: |
Ightm3.1(icre)Mbu |
| Name: |
immunoglobulin heavy chain complex; targeted mutation 3.1, Meinrad Busslinger |
| MGI ID: |
MGI:7539540 |
| Synonyms: |
IghCmuCre |
| Gene: |
Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic
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| Allele Type: |
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Targeted (Recombinase) |
| Mutation: |
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Insertion
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Ightm3.1(icre)Mbu expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Igh (MGI:96442) |
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Mutation details: The targeting vector consisted of a 4.1-kb long 50 homology region (containing Cmu1 to CmuS), a frt-flanked 2.1-kb DNA fragment containing the mouse phosphoglycerate kinase (Pgk1) promoter linked to the neomycin (Neo) resistance gene and a SV40 polyadenylation signal, a 1.6-kb DNA fragment containing the CmuM1/2 sequence linked in frame via the P2A peptide to an iCre gene followed by a 1.8-kb long 30 homology region was used to insert P2A-iCrE gene after the CmuM1/2 exons of the Igh gene . The frt-flanked Pgk1-Neo expression cassette was deleted by Flpe recombinase.
(J:341427)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Igh
(mouse)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Igh Mutation: |
44 strains or lines available
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| Original: |
J:341427 Hill L, et al., Enhancers of the PAIR4 regulatory module promote distal V(H) gene recombination at the Igh locus. EMBO J. 2023 Aug 1;42(15):e112741 |
| All: |
1 reference(s) |
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Analysis Tools
