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Mlc1tm2.1(cre/ERT2)Jhmc
Targeted Allele Detail
Summary
Symbol: Mlc1tm2.1(cre/ERT2)Jhmc
Name: megalencephalic leukoencephalopathy with subcortical cysts 1 homolog (human); targeted mutation 2.1, Joseph H McCarty
MGI ID: MGI:7540321
Synonyms: MLCT
Gene: Mlc1  Location: Chr15:88840087-88863192 bp, - strand  Genetic Position: Chr15, 44.46 cM
Alliance: Mlc1tm2.1(cre/ERT2)Jhmc page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:341452
Parent Cell Line:  Other (see notes) (ES Cell)
Strain of Origin:  129 x C57BL/6
Mutation
description
Allele Type:    Targeted (Inducible, Recombinase)
Inducer:    tamoxifen
Mutation:    Insertion
 
Mlc1tm2.1(cre/ERT2)Jhmc expression driven by 1 gene
 
Mutation details: The T2A-CreERT2 cassette was inserted immediately upstream of the endogenous stop codon in the exon 12 of the Mlc1 gene in frame. The FRT flanked Neo selection cassette was inserted 116 bp downstream of the 3' UTR. The Neo cassette in the targeting vector was removed during ES clone expansion. (J:341452)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Mlc1 (mouse)
Summary of all recombinase alleles driven by Mlc1.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Mlc1 Mutation:  28 strains or lines available
Notes
The targeting vector was transfected by electroporation of FLP 129/SvEv x C57Bl/6 (HF4) embryonic stem (ES) cells.
References
Original:  J:341452 Morales JE, et al., Mlc1-Expressing Perivascular Astrocytes Promote Blood-Brain Barrier Integrity. J Neurosci. 2022 Feb 23;42(8):1406-1416
All:  1 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory