Reporter:	lacZ
	Direct Detection

Structure	Level	Pattern	Image	Note
TS13: future brain neural fold	Present		1d

Structure	Cell Type	Level	Pattern	Image	Note
TS17: migrating neural crest	migratory neural crest cell	Present	Regionally restricted	1e	Prominent expression in neural crest cells migrating to and in the gut.
TS17: sensory ganglion		Present	Regionally restricted	1e	Expressed in sensory ganglia of the head and trunk.
TS17: sympathetic nervous system		Present		1e	Expressed in the primary sympathetic ganglion chain, or in cells localized along the developing axon tracts.
TS17: olfactory placode		Present		1e
TS17: otocyst		Present		1e
TS17: gut	migratory neural crest cell	Present		1e

Structure	Cell Type	Level	Pattern	Image	Note
TS20: nerve	Schwann cell precursor	Present		1f	Along peripheral nerves.
TS20: migrating neural crest	melanoblast	Present	Single cells	1f	Located subcutaneously.
TS20: cartilage condensation		Present		1f
TS20: central nervous system		Present		1f
TS20: dorsal root ganglion		Present		1f
TS20: inner ear		Present		1f
TS20: olfactory epithelium		Present		1f

Structure	Cell Type	Level	Pattern	Image	Note
TS20: central nervous system	oligodendrocyte precursor cell	Present

Structure	Level	Pattern	Image	Note
TS13: future brain neural fold	Present		1g	Similar as in the heterozygous.
TS13: migrating neural crest	Present		1g	Similar as in the heterozygous.

Structure	Cell Type	Level	Pattern	Image	Note
TS17: cranial ganglion		Present		1h	Reduced numbers of stained cells in cranial ganglia and along their projections, compared with the heterozygous.
TS17: gut	migratory neural crest cell	Absent		1h
TS17: sympathetic nervous system		Present		1h	Reduced numbers in the sympathetic primordium, compared with the heterozygous.

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present	Graded	1i	A rostro-caudal gradient in the staining intensity was seen, which was not present in the heterozygous.
TS20: nerve	Weak		1i	Severe reduction of positive cells along peripheral nerves, compared with the heterozygous.
TS20: cartilage condensation	Present		1i	Similar as in the heterozygous.
TS20: central nervous system	Present		1i	Similar as in the heterozygous.
TS20: inner ear	Present		1i	Similar as in the heterozygous.
TS20: olfactory epithelium	Present		1i	Similar as in the heterozygous.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: migrating neural crest	melanocyte	Weak			Severely reduced numbers of subcutaneous positive cells, compared with the control.