Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:128359
Oh HJ, et al., PIAS1 interacts with and represses SOX9 transactivation activity. Mol Reprod Dev. 2007 Nov;74(11):1446-55
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:7574628
|
Gene symbol:
|
Pias1
|
Gene name:
|
protein inhibitor of activated STAT 1
|
Antibody:
|
Anti-Pias1
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor 488
|
|
Results
|
Specimen
2A:
embryonic day 13.0
(more )
Note: Double labeled: green - Pias1; red - Sox9 (assay MGI:7574629).
Specimen
2A:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 13.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Pias1; red - Sox9 (assay MGI:7574629).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS21: Sertoli cell |
Sertoli cell |
Present |
|
2D
,
2A
,
2F
,
2C
|
Expression co-localized with Sox9. Pias1 was positive within a few dot-like structures in the Sertoli cells.
|
TS21: germ cell of testis |
|
Present |
|
2F
,
2D
,
2C
,
2A
|
|
TS21: mesonephros of male |
|
Present |
Ubiquitous |
2A
,
2D
,
2C
,
2F
|
Expression was ubiquitous in the nuclei of cells with the gonad and mesonephros.
|
TS21: testis |
|
Present |
Ubiquitous |
2A
,
2D
,
2C
,
2F
|
Expression was ubiquitous in the nuclei of cells with the gonad and mesonephros.
|
Specimen
3C:
embryonic day 15.0
(more )
Note: Double labeled: green - Pias1; red - Sox9 (assay MGI:7574629).
Specimen
3C:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 15.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Pias1; red - Sox9 (assay MGI:7574629).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: cartilage |
Present |
Ubiquitous |
3G
,
3D
,
3C
,
3F
|
Expression was ubiquitous in the cartilage primordium, and co-localized with Sox9 in dot-like structures within the positive nuclei.
|
|