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Gene Expression Data
Assay Details
Assay
Reference: J:87158 Fritz-Six KL, et al., Aberrant myofibril assembly in tropomodulin1 null mice leads to aborted heart development and embryonic lethality. J Cell Biol. 2003 Dec 8;163(5):1033-44
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7580878
Gene symbol: Myom1
Gene name: myomesin 1
Antibody: clone B4
Results
Specimen 9 E8-8.5 +/+: embryonic day 8.0-8.5 (more )
Note: Double labeled: red - Myom1; green - phallacidin.
Structure Cell Type Level Pattern Image Note
TS12: cardiac muscle tissue cardiac muscle cell Present 9_E8-8.5 merge +/+ , 9_E8-8.5 myomesin +/+ Staining was observed in a periodic dot-like or striped pattern along the myofibrils (depending on their thickness), where it colocalized with gaps in F-actin staining.

Specimen 9 E8-8.5 -/-: embryonic day 8.0-8.5; Tmod1tm1Vmf/Tmod1tm1Vmf (more )
Note: Double labeled: red - Myom1; green - phallacidin.
Structure Cell Type Level Pattern Image Note
TS12: cardiac muscle tissue cardiac muscle cell Present 9_E8-8.5 merge -/- , 9_E8-8.5 myomesin -/- In the mutant, short sets of periodic myomesin dots were frequently found located along structures with continuous F-actin staining. However, myomesin staining never became striated. Independent aggregates of myomesin and F-actin that did not colocalize were also observed.

Specimen 9 E9.5 +/+: embryonic day 9.5 (more )
Note: Double labeled: red - Myom1; green - phallacidin.
Structure Cell Type Level Pattern Image Note
TS15: cardiac muscle tissue cardiac muscle cell Present 9_E9.5 myomesin +/+ , 9_E9.5 merge +/+ In E9.5 wild-type embryos, striations of myomesin staining became increasingly obvious, due to lateral alignment of the myofibrils.

Specimen 9 E9.5 -/-: embryonic day 9.5; Tmod1tm1Vmf/Tmod1tm1Vmf (more )
Note: Double labeled: red - Myom1; green - phallacidin.
Structure Cell Type Level Pattern Image Note
TS15: cardiac muscle tissue cardiac muscle cell Present 9_E9.5 myomesin -/- , 9_E9.5 merge -/- In the mutant, short sets of periodic myomesin dots were frequently found located along structures with continuous F-actin staining. However, myomesin staining never became striated. Independent aggregates of myomesin and F-actin that did not colocalize were also observed.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
06/12/2024
MGI 6.13
The Jackson Laboratory