Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:291486
Daniel E, et al., Cyp26b1 is an essential regulator of distal airway epithelial differentiation during lung development. Development. 2020 Feb 20;147(4):dev181560
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:7623741
|
Gene symbol:
|
Cyp26b1
|
Gene name:
|
cytochrome P450, family 26, subfamily b, polypeptide 1
|
Probe:
|
MGC clone 67317
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Fluorescein
|
|
Results
|
Specimen
1D:
embryonic day 15.5
(more )
Note: Double labeled: green - Cyp26b1; red - Emcn (assay MGI:7623742) and Pecam1 (assay MGI:7623743).
Specimen
1D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Cyp26b1; red - Emcn (assay MGI:7623742) and Pecam1 (assay MGI:7623743).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS23: lung |
lung endothelial cell |
Present |
Punctate |
1D
|
Co-expression was detected with endothelial cell markers Pecam1/Emcn.
|
TS23: lung epithelium |
|
Weak |
Punctate |
1D
|
Immunopositive punctae were observed in some epithelial and stromal regions, but at much lower levels compared with endothelial cells.
|
TS23: lung |
stromal cell |
Weak |
Punctate |
1D
|
Immunopositive punctae were observed in some epithelial and stromal regions, but at much lower levels compared with endothelial cells.
|
Specimen
1H:
embryonic day 15.5
(more )
Note: Double labeled: green - Cyp26b1; red - Emcn (assay MGI:7623742) and Pecam1 (assay MGI:7623743).
Specimen
1H:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Cyp26b1; red - Emcn (assay MGI:7623742) and Pecam1 (assay MGI:7623743).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS23: metanephros |
endothelial cell |
Present |
Punctate |
1H
|
Co-expression was detected with endothelial cell markers Pecam1/Emcn.
|
TS23: metanephros epithelium |
|
Weak |
Punctate |
1H
|
Immunopositive punctae were observed in some epithelial and stromal regions, but at much lower levels compared with endothelial cells.
|
TS23: metanephros |
stromal cell |
Weak |
Punctate |
1H
|
Immunopositive punctae were observed in some epithelial and stromal regions, but at much lower levels compared with endothelial cells.
|
Specimen
5G:
embryonic day 18.5
(more )
Note: Double labeled: green - Cyp26b1; red - Emcn (assay MGI:7623742).
Specimen
5G:
(close )
Genetic Background:
|
involves: C57BL/6J * CD-1
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Cyp26b1; red - Emcn (assay MGI:7623742).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: lung |
lung endothelial cell |
Present |
Punctate |
5G
|
Co-expression was detected with endothelial cell marker Emcn.
|
Specimen
5H:
(close )
Genetic Background:
|
involves: C57BL/6J * CD-1
|
Mutant Allele(s):
|
Cyp26b1em1Oncl/Cyp26b1em1Oncl
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Cyp26b1; red - Emcn (assay MGI:7623742).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: lung |
lung endothelial cell |
Strong |
Punctate |
5H
|
Expression was strongly upregulated in mutant endothelial cells co-expressing Emcn.
|
Specimen
S2F:
embryonic day 18.5
(more )
Note: Double labeled: green - Cyp26b1; red - Lyve1 (assay MGI:7623744).
Specimen
S2F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Cyp26b1; red - Lyve1 (assay MGI:7623744).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: lung |
endothelial cell of lymphatic vessel |
Present |
|
S2F'
,
S2F
|
Co-expression with Lyve1 revealed that Cyp26b1 is also expressed in lymphatic endothelium in the lung.
|
|