Mecp2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:74625 Shahbazian MD, et al., Insight into Rett syndrome: MeCP2 levels display tissue- and cell-specific differences and correlate with neuronal maturation. Hum Mol Genet. 2002 Jan 15;11(2):115-24
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7628659
Gene symbol:	Mecp2
Gene name:	methyl CpG binding protein 2

Antibody:	anti-Mecp2
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Results

Specimen 3A: embryonic day 16.5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS24: cerebral cortex marginal layer		Strong	Single cells	3C , 3B , 3A	Expression is highest in the Cajal-Retzius neurons.
TS24: cerebral cortex	Cajal-Retzius cell	Strong	Regionally restricted	3A , 3C , 3B	Expression is highest in the Cajal-Retzius neurons of the marginal zone in the deeper layer and is absent in the more superficial, immature cortical neurons below the Cjal-Retzius cells.

Specimen 3D: embryonic day 18.5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS26: cerebral cortex	neuron	Strong	Non-Uniform	3E , 3D , 3F	Expression is stronger in the deeper layers.

Specimen 4A: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	4A	Expression is in the cerebral cortex.

Specimen 4B: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present		4B

Specimen 4C: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present		4C

Specimen 4D: embryonic day 17.5 (more

)

Structure	Level	Pattern	Image	Note
TS25: spinal cord	Present		4D

Specimen 5A: postnatal week 19 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present		5E , 5A

Specimen 5B: postnatal week 19 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		5B , 5F

Specimen 5C: postnatal week 19 (more

)

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		5C , 5G

Specimen 5D: postnatal week 19 (more

)

Structure	Level	Pattern	Image	Note
TS28: pons	Present		5D , 5H

Specimen Table 1 E10.5: embryonic day 10.5 (more

)

Structure	Cell Type	Level	Pattern	Note
TS17: brain marginal layer	neuron	Trace	Single cells	There is trace expression in some neurons and is absent in the deep and superciial layers of the cerebral cortex.
TS17: hindbrain		Absent		Absent in the pons and the medulla oblongata.
TS17: future spinal cord		Absent

Specimen Table 1 E11.5: embryonic day 11.5 (more

)

Structure	Cell Type	Level	Pattern	Note
TS19: brain marginal layer	neuron	Present	Single cells	Expression is in some neurons and is absent in the deep and superciial layers of the cerebral cortex.
TS19: metencephalon basal plate	neuron	Present	Single cells	Expression is in some neurons of the pons.
TS19: hindbrain	neuron	Present	Single cells	Expression is in some neurons of the medulla oblongata.
TS19: future spinal cord	neuron	Present	Single cells	Expression is in some neurons in the spinal cord.

Specimen Table 1 E13.5: embryonic day 13.5 (more

)

Structure	Cell Type	Level	Pattern	Note
TS21: brain marginal layer	neuron	Present		Expression is in the majority of neurons.
TS21: telencephalon	neuron	Present	Single cells	Expression is in some neurons in the caudate-putamen.
TS21: medulla oblongata	neuron	Trace	Single cells	There is trace expression in some neurons.
TS21: pons	neuron	Trace	Single cells	There is trace expression in some neurons.
TS21: spinal cord	neuron	Present	Single cells	Expression is in some neurons.
TS21: cerebral cortex		Absent
TS21: thalamus		Absent

Specimen Table 1 E14.5: embryonic day 14.5 (more

)

Structure	Cell Type	Level	Pattern	Note
TS22: brain marginal layer	neuron	Present		Expression is in the majority of neurons.
TS22: thalamus	neuron	Present		Expression is in the majority of neurons.
TS22: cerebral cortex	neuron	Present	Regionally restricted	Expression is in some neurons in the deep cortical layers, absent in the superficial layers.
TS22: caudate-putamen	neuron	Present	Regionally restricted	Expression is in some neurons.
TS22: cerebellum	neuron	Present	Regionally restricted	Expression is in some neurons.
TS22: medulla oblongata	neuron	Present	Regionally restricted	Expression is in some neurons.
TS22: pons	neuron	Present	Regionally restricted	Expression is in some neurons.
TS22: hypothalamus		Absent

Specimen table 1 E16.5: embryonic day 16.5 (more

)

Structure	Cell Type	Level	Pattern	Note
TS24: brain marginal layer	neuron	Present		Expression is in the majority of neurons.
TS24: medulla oblongata	neuron	Present		Expression is in the majority of neurons.
TS24: thalamus	neuron	Present		Expression is in the majority of neurons.
TS24: cerebral cortex	neuron	Present	Regionally restricted	Expression is in the majority of neurons in the deep cortical layers, absent in the superficial layers.
TS24: hippocampus	neuron	Present	Regionally restricted	Expression is in some neurons.
TS24: spinal cord	neuron	Present	Regionally restricted	Expression is in some neurons.
TS24: caudate-putamen	neuron	Trace	Regionally restricted	There is trace expression in some neurons.
TS24: cerebellum	neuron	Trace	Regionally restricted	There is trace expression in some neurons.
TS24: deep cerebellar nucleus	neuron	Trace	Regionally restricted	There is trace expression in some neurons.
TS24: hypothalamus	neuron	Trace	Regionally restricted	There is trace expression in some neurons.
TS24: pons	neuron	Trace	Regionally restricted	There is trace expression in some neurons.

Specimen Table1 E17.5: embryonic day 17.5 (more

)

Structure	Cell Type	Level	Pattern	Note
TS25: brain marginal layer	neuron	Present		Expression is in the majority of neurons.
TS25: cerebellum	neuron	Present		Expression is in the majority of neurons.
TS25: deep cerebellar nucleus	neuron	Present		Expression is in the majority of neurons.
TS25: medulla oblongata	neuron	Present		Expression is in the majority of neurons.
TS25: pons	neuron	Present		Expression is in the majority of neurons.
TS25: thalamus	neuron	Present		Expression is in the majority of neurons.
TS25: cerebral cortex	neuron	Present		Expression is in the majority of neurons in the deep cortical layers, absent in the superficial layers.
TS25: hippocampus	neuron	Present		Expression is in some neurons.
TS25: caudate-putamen	neuron	Trace	Single cells	There is trace expression in some neurons.
TS25: hypothalamus	neuron	Trace	Single cells	There is trace expression in some neurons.
TS25: spinal cord	neuron	Trace	Single cells	There is trace expression in some neurons.

Specimen Table 1 E18.5: embryonic day 18.5 (more

)

Structure	Cell Type	Level	Pattern	Note
TS26: brain marginal layer	neuron	Present		Expression is in a majority of neurons.
TS26: caudate-putamen	neuron	Present		Expression is in a majority of neurons.
TS26: cerebellum	neuron	Present		Expression is in a majority of neurons.
TS26: cerebral cortex	neuron	Present		Expression is in a majority of neurons.
TS26: deep cerebellar nucleus	neuron	Present		Expression is in a majority of neurons.
TS26: medulla oblongata	neuron	Present		Expression is in a majority of neurons.
TS26: pons	neuron	Present		Expression is in a majority of neurons.
TS26: spinal cord	neuron	Present		Expression is in a majority of neurons.
TS26: thalamus	neuron	Present		Expression is in a majority of neurons.
TS26: hippocampus	neuron	Present	Single cells	Expression is in a some of neurons.
TS26: hypothalamus	neuron	Present	Single cells	Expression is in a some of neurons.

Specimen Table 1 P10: postnatal day 10 (more

)

Structure	Cell Type	Level	Note
TS28: brain marginal layer	neuron	Present	Expression is in a majority of neurons.
TS28: caudate-putamen	neuron	Present	Expression is in a majority of neurons.
TS28: cerebellum	neuron	Present	Expression is in a majority of neurons.
TS28: cerebral cortex	neuron	Present	Expression is in a majority of neurons.
TS28: deep cerebellar nucleus	neuron	Present	Expression is in a majority of neurons.
TS28: hippocampus	neuron	Present	Expression is in a majority of neurons.
TS28: hypothalamus	neuron	Present	Expression is in a majority of neurons.
TS28: medulla oblongata	neuron	Present	Expression is in a majority of neurons.
TS28: pons	neuron	Present	Expression is in a majority of neurons.
TS28: thalamus	neuron	Present	Expression is in a majority of neurons.

Specimen Table 1 7 weeks: postnatal week 7 (more

)

Structure	Cell Type	Level	Note
TS28: brain marginal layer	neuron	Present	Expression is in a majority of neurons.
TS28: caudate-putamen	neuron	Present	Expression is in a majority of neurons.
TS28: cerebellum	neuron	Present	Expression is in a majority of neurons.
TS28: cerebral cortex	neuron	Present	Expression is in a majority of neurons.
TS28: deep cerebellar nucleus	neuron	Present	Expression is in a majority of neurons.
TS28: hippocampus	neuron	Present	Expression is in a majority of neurons.
TS28: hypothalamus	neuron	Present	Expression is in a majority of neurons.
TS28: medulla oblongata	neuron	Present	Expression is in a majority of neurons.
TS28: pons	neuron	Present	Expression is in a majority of neurons.
TS28: thalamus	neuron	Present	Expression is in a majority of neurons.

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