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Gene Expression Data
Assay Details
Assay
Reference: J:166908 Sasaki T, et al., Regulation of hematopoietic cell clusters in the placental niche through SCF/Kit signaling in embryonic mouse. Development. 2010 Dec;137(23):3941-52
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7641776
Gene symbol: Cd34
Gene name: CD34 antigen
Antibody: anti-CD34
Detection system: Secondary antibody coupled to Alexa Fluor 568
Results
Specimen 1B: embryonic day 10.5 (more )
Note: Double labeled: red - Cd34; green - Kit (assay MGI:7641777).
Structure Cell Type Level Pattern Image Note
TS17: labyrinthine zone placental villus capillary endothelial cell Present 1B merge , 1B CD34
TS17: labyrinthine zone hematopoietic cell Present Clusters 1B merge , 1B CD34 Positive clusters were attached to the endothelial wall of capillary vessels. Coexpressed with Kit.
Specimen 1C: embryonic day 10.5 (more )
Note: Aorta-gonadmesonephros (AGM) region. Double labeled: red - Cd34; green - Kit (assay MGI:7641777).
Structure Cell Type Level Pattern Image Note
TS17: hematopoietic system hematopoietic cell Present Clusters 1C merge , 1C CD34 Positive clusters were attached to the endothelial wall of the aorta. Coexpressed with Kit.
Specimen 1D Runx1-/-: embryonic day 10.5; Runx1tm1Spe/Runx1tm1Spe (more )
Note: Double labeled: red - Cd34; green - Kit (assay MGI:7641777).
Structure Cell Type Level Pattern Image Note
TS17: labyrinthine zone hematopoietic cell Absent 1D Runx1-/- No positive clusters were observed inside capillary vessels.
TS17: labyrinthine zone placental villus capillary endothelial cell Present 1D Runx1-/-
Specimen 1D Evi-1-/-: embryonic day 10.5; Mecomtm1Mmor/Mecomtm1Mmor (more )
Note: Double labeled: red - Cd34; green - Kit (assay MGI:7641777).
Structure Cell Type Level Pattern Image Note
TS17: labyrinthine zone hematopoietic cell Absent 1D Evi-1-/- No positive clusters were observed inside capillary vessels at the time of abnormal vessel formation.
TS17: labyrinthine zone placental villus capillary endothelial cell Present 1D Evi-1-/- Abnormal vessel formation was seen.
Specimen 1D c-Myb-/-: embryonic day 10.5; Mybtm1Ssp/Mybtm1Ssp (more )
Note: Double labeled: red - Cd34; green - Kit (assay MGI:7641777).
Structure Cell Type Level Pattern Image Note
TS17: labyrinthine zone hematopoietic cell Present Clusters 1D c-Myb-/- Positive cluster size overall was larger than that seen in wild-type embryos.
Specimen 4A c-Kit/CD34 left: embryonic day 9.5 (more )
Note: Double labeled: red - Cd34; green - Kit (assay MGI:7641777).
Structure Cell Type Level Pattern Image Note
TS15: allantois hematopoietic cell Present Clusters 4A-3 Coexpressed with Kit.
Specimen 4A c-Kit/CD34 right: embryonic day 9.5 (more )
Note: Double labeled: red - Cd34; green - Kit (assay MGI:7641777).
Structure Cell Type Level Pattern Image Note
TS15: chorionic plate hematopoietic cell Present Clusters 4A-4 Coexpressed with Kit.
Specimen 6G/H: embryonic day 11.5 (more )
Note: Double labeled: red - Cd34; green - Kitl (assay MGI:7641855).
Structure Cell Type Level Pattern Image Note
TS19: labyrinthine zone placental villus capillary endothelial cell Present 6H CD34 , 6G , 6H merge
Specimen S2: embryonic day 8.5 (more )
Note: Double labeled: red - Cd34; green - Kit (assay MGI:7641777).
Structure Cell Type Level Pattern Image Note
TS13: allantois hematopoietic cell Absent S2_left , S2_merge , S2_CD34 No positive clusters were observed.
TS13: chorion hematopoietic cell Absent S2_left No positive clusters were observed.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
MGI 6.13 		The Jackson Laboratory