Gene Expression Data
Assay Details
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Assay
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Reference:
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J:47841
Varlet I, et al., Nodal signaling and axis formation in the mouse. Cold Spring Harb Symp Quant Biol. 1997;62:105-13
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:7643429
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Gene symbol:
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Nodal
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Gene name:
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nodal growth differentiation factor
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
not shown:
embryonic day 3.5; Nodaltm1Rob/Nodal +
(more  )
Note: Blastocyst stage.
Specimen
not shown:
(close  )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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4% Paraformaldehyde
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Note:
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Blastocyst stage.
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Specimen
1A:
embryonic day 5.5; Nodaltm1Rob/Nodal +
(more )
Specimen
1A:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 5.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS7: epiblast |
Weak |
Widespread |
1A
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Low level of expression throughout epiblast and visceral endoderm.
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| TS7: primitive endoderm |
Weak |
Widespread |
1A
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Low level of expression throughout epiblast and visceral endoderm.
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Specimen
1C:
embryonic day 6.0; Nodaltm1Rob/Nodal +
(more )
Specimen
1C:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 6.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS8: epiblast |
Strong |
Regionally restricted |
1C
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Higher levels of expression broadly distributed in epiblast adjacent to extraembryonic junction.
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| TS8: primitive endoderm |
Weak |
Ubiquitous |
1C
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Low levels of expression throughout visceral endoderm.
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Specimen
1D:
embryonic day 6.0; Nodaltm1Rob/Nodal +
(more )
Specimen
1D:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 6.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS8: egg cylinder |
Present |
Regionally restricted |
1D
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Expression becomes localized to presumptive posterior side.
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| TS8: primitive endoderm |
Weak |
Uniform |
1D
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Low levels of expression throughout visceral enddoderm.
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| TS8: epiblast |
Present |
Regionally restricted |
1D
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Expression in anterior epiblast.
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Specimen
1E:
embryonic day 6.0; Nodaltm1Rob/Nodal +
(more )
Specimen
1E:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 6.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS8: egg cylinder |
Present |
Regionally restricted |
1E
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Expression becomes localized to presumptive posterior side.
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| TS8: primitive endoderm |
Weak |
Uniform |
1E
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Low levels of expression throughout visceral enddoderm.
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| TS8: epiblast |
Present |
Regionally restricted |
1E
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Expression in anterior epiblast.
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Specimen
2A (left):
embryonic day 7.5; Nodaltm1Rob/Nodal +
(more )
Note: Double labeled: blue - Nodal reporter; brown - Shh (assay MGI:7643430).
Specimen
2A (left):
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: blue - Nodal reporter; brown - Shh (assay MGI:7643430).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: primitive streak |
Present |
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2A (left)
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Expression progressively dowregulated in primitive streak.
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Specimen
2A (center):
embryonic day 7.5; Nodaltm1Rob/Nodal +
(more )
Note: Double labeled: blue - Nodal reporter; brown - Shh (assay MGI:7643430).
Specimen
2A (center):
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: blue - Nodal reporter; brown - Shh (assay MGI:7643430).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: primitive streak |
Present |
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2A (center)
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Expression progressively dowregulated in primitive streak.
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Specimen
2A (right):
embryonic day 7.5; Nodaltm1Rob/Nodal +
(more )
Note: Head fold stage. Double labeled: blue - Nodal reporter; brown - Shh (assay MGI:7643430).
Specimen
2A (right):
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Head fold stage. Double labeled: blue - Nodal reporter; brown - Shh (assay MGI:7643430).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: node |
Present |
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2A (right)
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Expression beomes localized to the node in headfold stage embryo.
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Specimen
2B:
embryonic day 7.0; Nodaltm1Rob/Nodal +
(more )
Note: Late streak stage. Double labeled: blue - Nodal reporter; brown - Shh (assay MGI:7643430).
Specimen
2B:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 7.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Late streak stage. Double labeled: blue - Nodal reporter; brown - Shh (assay MGI:7643430).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS10: primitive streak |
Present |
Regionally restricted |
2B
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Expression in distal tip of primitive streak during initial stages of node formation.
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Specimen
2C:
embryonic day 7.5; Nodaltm1Rob/Nodal +
(more )
Note: Head fold stage. Double labeled: blue - Nodal reporter; brown - Shh (assay MGI:7643430).
Specimen
2C:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 7.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Head fold stage. Double labeled: blue - Nodal reporter; brown - Shh (assay MGI:7643430).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: notochordal plate |
Present |
Regionally restricted |
2C
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Expression at periphery of notochordal plate in a domain adjacent to and partially overlapping with Shh expression.
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Specimen
2D:
embryonic day 7.0; Nodaltm1Rob/Nodal +
(more )
Note: Late streak stage.
Specimen
2D:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 7.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Late streak stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS10: embryo mesoderm |
Present |
Regionally restricted |
2D
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Expression in notochordal plate.
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Specimen
2E:
embryonic day 7.5; Nodaltm1Rob/Nodal +
(more )
Note: Presomite stage.
Specimen
2E:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 7.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Presomite stage.
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Specimen
3A:
embryonic day 8.0; Nodaltm1Rob/Nodal +
(more )
Note: 4-somite stage.
Specimen
3A:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
3A
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present / RR Asymmetric expression in lateral plate mesoderm. Expression in band of mesodermal cells on left side of embryo, with rostral limit at position of forming heart.
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Specimen
3B:
embryonic day 8.0; Nodaltm1Rob/Nodal +
(more )
Note: 4-somite stage.
Specimen
3B:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: somatopleure |
Present |
Regionally restricted |
3B
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Expression in somatic and splanchnic mesoderm on left side of embryo.
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| TS12: splanchnopleure |
Present |
Regionally restricted |
3B
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Expression in somatic and splanchnic mesoderm on left side of embryo.
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Specimen
3C:
embryonic day 8.5; Nodaltm1Rob/Nodal +
(more )
Specimen
3C:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: embryo mesoderm |
Present |
Regionally restricted |
3C
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Following embryonic turning, expression not detectable in lateral plate mesoderm.
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| TS14: tail bud |
Present |
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3C
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Expression retained in cells of tail bud
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Specimen
3D:
embryonic day 9.5; Nodaltm1Rob/Nodal +
(more )
Specimen
3D:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: telencephalon |
Present |
Regionally restricted |
3D
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Expression confined to dorsal midline of telencephalon.
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| TS15: branchial arch |
Present |
Regionally restricted |
3D
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Expression in small group of cells between 1st and 2nd branchial arches.
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Specimen
6A (left):
embryonic day 8.5; Nodaltm1Rob/Nodal +
(more )
Note: 8-12 somite stage.
Specimen
6A (left):
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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8-12 somite stage.
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Specimen
6A (center):
embryonic day 8.5; Foxa2tm1Jrt/Foxa2 +, Nodaltm1Rob/Nodal +
(more )
Note: 8-12 somite stage.
Specimen
6A (center):
(close )
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Genetic Background:
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involves: 129/Sv * 129X1/SvJ
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Mutant Allele(s):
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Foxa2tm1Jrt/Foxa2+
Nodaltm1Rob/Nodal+
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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8-12 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: lateral plate mesoderm |
Present |
Regionally restricted |
6A (center)
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Bilateral expression in lateral plate mesoderm.
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Specimen
6A (right):
embryonic day 8.5; Foxa2tm1Jrt/Foxa2 +, Nodaltm1Rob/Nodal +
(more )
Note: 8-12 somite stage.
Specimen
6A (right):
(close )
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Genetic Background:
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involves: 129/Sv * 129X1/SvJ
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Mutant Allele(s):
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Foxa2tm1Jrt/Foxa2+
Nodaltm1Rob/Nodal+
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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8-12 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: lateral plate mesoderm |
Present |
Regionally restricted |
6A (right)
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Bilateral expression in lateral plate mesoderm.
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Analysis Tools