Antibody:	anti-GLAST
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS15: future spinal cord	Absent

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Regionally restricted	4A	Authors report expression in ventral cord. Faint expression in radially oriented fibrous processes running through ventricular zone and ventral horn reaching pial surface.
TS19: neural tube ventricular layer	Present	Regionally restricted	4A	Higher levels associated with floor plate and ventricular side of lateral wall. Little or low expression in a narrow ventrobasal region adjacent to floor plate.

Structure	Level	Pattern	Image	Note
TS21: spinal cord floor plate	Strong		4B	Expression prominently increased in ventral cord, including floor plate, and ventricular zone.
TS21: spinal cord ventral horn	Strong		4B	Expression prominently increased in ventral cord, including floor plate, and ventricular zone.
TS21: spinal cord ventricular layer	Strong		4B	Expression prominently increased in ventral cord, including floor plate, and ventricular zone.
TS21: spinal cord mantle layer	Present	Regionally restricted	4B	Expression in radial processes, giving off short circumferential branches between mantle and marginal zones.
TS21: spinal cord marginal layer	Present	Regionally restricted	4B	Expression in radial processes, giving off short circumferential branches between mantle and marginal zones.
TS21: spinal cord dorsal horn	Weak	Regionally restricted	4B	In dorsal cord, low expression in ventricular zone and radial processes.

Structure	Level	Pattern	Image	Note
TS23: spinal cord ventricular layer	Present		4C	Expression lowered in ventricular zone.
TS23: spinal cord floor plate	Strong		4C	Intense expression in floor plate.
TS23: spinal cord dorsal horn	Present	Regionally restricted	4C	Intense expression in radial process of both ventral and dorsal cords.
TS23: spinal cord ventral horn	Present	Regionally restricted	4C	Intense expression in radial process of both ventral and dorsal cords.

Structure	Level	Pattern	Image	Note
TS26: spinal cord gray matter	Present	Regionally restricted	4D	Expression in punctate/reticular structures increasing in number and predominating throughout gray matter.
TS26: spinal cord floor plate	Trace		4D	Expression diminished or disappeared from floor plate, ventricular zone (ependymal layer), radial processes and marginal zone (white matter).
TS26: spinal cord marginal layer	Trace		4D	Expression diminished or disappeared from floor plate, ventricular zone (ependymal layer), radial processes and marginal zone (white matter).
TS26: spinal cord ventricular layer	Trace		4D	Expression diminished or disappeared from floor plate, ventricular zone (ependymal layer), radial processes and marginal zone (white matter).

Structure	Level	Pattern	Image	Note
TS27: spinal cord marginal layer	Trace		5A	Expression diminished or disappeared from floor plate, ventricular zone (ependymal layer), radial processes and marginal zone (white matter).

Structure	Level	Pattern	Image	Note
TS28: spinal cord ependymal layer	Trace		5B	Expression diminished or disappeared from floor plate, ventricular zone (ependymal layer), radial processes and marginal zone (white matter).
TS28: spinal cord marginal layer	Trace		5B	Expression diminished or disappeared from floor plate, ventricular zone (ependymal layer), radial processes and marginal zone (white matter).

Structure	Level	Pattern	Image	Note
TS28: spinal cord gray matter	Strong	Widespread	5C	Strong expression thoughout gray matter. Increased expression in neuropil region.

Structure	Level	Pattern	Image	Note
TS28: spinal cord gray matter	Strong	Widespread	5D	Strong expression thoughout gray matter. Increased expression in neuropil region.

Structure	Level	Pattern	Image	Note
TS28: spinal cord gray matter	Trace		5E	Expression disappears from most regions of gray matter.
TS28: spinal cord dorsal horn	Trace	Regionally restricted	5E	Low to moderate levels of expression in superficial layers of dorsal horn and around central canal.
TS28: spinal cord ependymal layer	Trace	Regionally restricted	5E	Low to moderate levels of expression in superficial layers of dorsal horn and around central canal.