Gene Expression Data
Assay Details
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Assay
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Reference:
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J:44290
Shibata T, et al., Glutamate transporter GLAST is expressed in the radial glia-astrocyte lineage of developing mouse spinal cord. J Neurosci. 1997 Dec 1;17(23):9212-9
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7643801
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Gene symbol:
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Slc1a3
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Gene name:
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solute carrier family 1 (glial high affinity glutamate transporter), member 3
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Antibody:
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anti-GLAST
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
6A:
embryonic day 13.5
(more  )
Note: Double labeled: green - Slc1a3; red - propidium iodide.
Specimen
6A:
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Slc1a3; red - propidium iodide.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord ventricular layer |
Present |
Regionally restricted |
6A
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Expression surrounding most of cell bodies in ventricular zone, which were elongated in shape and formed pseudostratified neuroepithelium.
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| TS21: spinal cord mantle layer |
Present |
Regionally restricted |
6A
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Expression in radial processes, smooth in countour and traversing mantle zone between rows of neuronal cells bodies.
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Specimen
6B:
embryonic day 13.5
(more )
Note: Double labeled: green - Slc1a3; red - Nes (assay MGI:7643802).
Specimen
6B:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Slc1a3; red - Nes (assay MGI:7643802).
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Specimen
6C:
embryonic day 15.5
(more )
Note: Double labeled: green - Slc1a3; red - Nes (assay MGI:7643802).
Specimen
6C:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Slc1a3; red - Nes (assay MGI:7643802).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: spinal cord mantle layer |
Present |
Regionally restricted |
6C
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Expression in punctate or irregular protrusions from radial processes. Expression in shafts of radiall processes.
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Specimen
6D:
embryonic day 15.5
(more )
Note: Double labeled: green - Slc1a3; red - Nes (assay MGI:7643802).
Specimen
6D:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Slc1a3; red - Nes (assay MGI:7643802).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: spinal cord marginal layer |
Present |
Regionally restricted |
6D
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Expression in conical endfeet and in punctate or irregular protrusions from radial processes. Expression in shafts of radial processes.
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Specimen
6F:
postnatal day 7
(more )
Note: Double labeled: green - Slc1a3; red - Gfap (assay MGI:7643804).
Specimen
6F:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Slc1a3; red - Gfap (assay MGI:7643804).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spinal cord |
Present |
Regionally restricted |
6F
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Expression in punctate/reticular structures distributed around Gfap-expressing fibers. Little overlap in expression with Gfap.
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Specimen
6G:
postnatal day 7
(more )
Note: Double labeled: green - Slc1a3; red - Syp (assay MGI:7643805).
Specimen
6G:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Slc1a3; red - Syp (assay MGI:7643805).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spinal cord |
Present |
Regionally restricted |
6G
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Expression in punctate/reticular structures closely apposed to nerve terminals.
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Analysis Tools