Antibody:	Anti-PLCe
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Non-Uniform	3Ba , 3Bc , 3Bf , 3Bd	Expression was weak in almost all the regions of the neuroepithelium surrounding the fourth ventricle. Among this uniform expression, cells in the outer-most zone stood out with a markedly high level of expression. Almost all highly expressing cells were negative for Nestin. In contrast, all other neuroepithelial cells were positive for Nestin.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Non-Uniform	3Bl , 3Bi , 3Bg , 3Bj	Expression was weak in almost all the regions of the neuroepithelium surrounding the fourth ventricle. Among this uniform expression, cells in the outer-most zone stood out with a markedly high level of expression. Almost all highly expressing cells were strongly positive for Map2. In contrast, almost all the other neuroepithelial cells were negative for MAP2.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Weak	Uniform	3C merge top , 3C PLCe top	The weak, uniform expression was almost uniformly co-stained with Nestin, except for the outermost zone, which exhibited stronger Nestin staining.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Weak	Uniform	3C PLCe bottom , 3C merge bottom

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present	Non-Uniform	4Ad , 4Af , 4Ac , 4Aa	High expressing cells were detected in the marginal zone, although uniform and weak expression was observed in the region from the intermediate zone to the marginal zone.
TS22: telencephalon marginal layer	Present		4Aa , 4Ac , 4Ad , 4Af	None of the highly-positive cells co-localized with Nestin.

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present	Non-Uniform	4Aj , 4Al , 4Ag , 4Ai	High expressing cells were detected in the marginal zone, although uniform and weak expression was observed in the region from the intermediate zone to the marginal zone.
TS22: telencephalon marginal layer	Present		4Aj , 4Ag , 4Al , 4Ai	Highly expressing cells almost coincided with the Map2-positive cells.

Structure	Level	Pattern	Image	Note
TS22: neural retina	Present		4B

Structure	Level	Pattern	Image	Note
TS22: thalamus	Strong		7D merge left , 7D PLCe left	Almost all the Map2-positive cells were strongly positive for Plce1.

Structure	Level	Pattern	Image	Note
TS22: thalamus	Present		7D merge right , 7D PLCe right	Positively stained cells were negative for Gfap.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		7E merge left , 7E PLCe left	Almost all the Map2-positive cells were strongly positive for Plce1.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		7E PLCe right , 7E merge right	Positively stained cells were negative for Gfap.