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Gene Expression Data
Assay Details
Assay
Reference: J:84137 Wu D, et al., Neuronal lineage-specific induction of phospholipase Cepsilon expression in the developing mouse brain. Eur J Neurosci. 2003 Apr;17(8):1571-80
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7645215
Gene symbol: Map2
Gene name: microtubule-associated protein 2
Antibody: anti-MAP2 (2a+2b+2c; clone HM-2)
Detection system: Secondary antibody coupled to rhodamine
Results
Specimen 3Bh: embryonic day 10.5 (more )
Note: Fix - 3.7% paraformaldehyde. Double labeled: red - Map2; green - Plce1 (assay MGI:7645206).
Structure Cell Type Level Pattern Image Note
TS17: hindbrain CNS neuron (sensu Vertebrata) Strong Regionally restricted 3Bh , 3Bi , 3Bl , 3Bk Expression was detected in differentiated neurons. Strong co-labeling was observed with the highly expressing Plce1 cells.
Specimen 3C: embryonic day 10.5 (more )
Note: Fix - 3.7% paraformaldehyde. Double labeled: red - Map2; green - Plce1 (assay MGI:7645206).
Structure Level Pattern Image Note
TS17: telencephalon Absent 3C merge bottom , 3C MAP2
Specimen 4Ah: embryonic day 14.5 (more )
Note: Fix - 3.7% paraformaldehyde. Double labeled: red - Map2; green - Plce1 (assay MGI:7645206).
Structure Cell Type Level Pattern Image Note
TS22: pia mater Strong 4Ah , 4Ai , 4Al , 4Ak
TS22: telencephalon marginal layer CNS neuron (sensu Vertebrata) Present 4Al , 4Ai , 4Ak , 4Ah Expression almost coincided with the Plce1-highly positive cells.
Specimen 7D: embryonic day 14.5 (more )
Note: Fix - 3.7% paraformaldehyde. Double labeled: red - Map2; green - Plce1 (assay MGI:7645206).
Structure Level Pattern Image Note
TS22: thalamus Present 7D Map2 , 7D merge left Almost all of the Map2-positive cells were strongly positive for Plce1.
Specimen 7E: postnatal adult (more )
Note: Fix - 3.7% paraformaldehyde. Double labeled: red - Map2; green - Plce1 (assay MGI:7645206).
Structure Level Pattern Image Note
TS28: cerebral cortex Present 7E Map2 , 7E merge left Almost all of the Map2-positive cells were strongly positive for Plce1.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
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